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Psychopharmacology

, Volume 234, Issue 7, pp 1069–1077 | Cite as

Bi-phasic regulation of glycogen content in astrocytes via Cav-1/PTEN/PI3K/AKT/GSK-3β pathway by fluoxetine

  • Qiufang Bai
  • Dan Song
  • Li Gu
  • Alexei Verkhratsky
  • Liang Peng
Original Investigation

Abstract

Objective

Here, we present the data indicating that chronic treatment with fluoxetine regulates Cav-1/PTEN/PI3K/AKT/GSK-3β signalling pathway and glycogen content in primary cultures of astrocytes with bi-phasic concentration dependence.

Results

At lower concentrations, fluoxetine downregulates gene expression of Cav-1, decreases membrane content of PTEN, increases activity of PI3K/AKT, and elevates GSK-3β phosphorylation thus suppressing its activity. At higher concentrations, fluoxetine acts in an inverse fashion. As expected, fluoxetine at lower concentrations increased while at higher concentrations decreased glycogen content in astrocytes.

Conclusions

Our findings indicate that bi-phasic regulation of glycogen content via Cav-1/PTEN/PI3K/AKT/GSK-3β pathway by fluoxetine may be responsible for both therapeutic and side effects of the drug.

Keywords

Fluoxetine Cav-1 PTEN PI3K/AKT GSK-3β Glycogen 

Notes

Acknowledgements

This study was supported by Grant No. 31400925 to DS from the National Natural Science Foundation of China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Qiufang Bai
    • 1
  • Dan Song
    • 1
  • Li Gu
    • 1
  • Alexei Verkhratsky
    • 2
    • 3
  • Liang Peng
    • 1
  1. 1.Laboratory of Metabolic Brain Diseases, Institute of Metabolic Disease Research and Drug DevelopmentChina Medical UniversityShenyangPeople’s Republic of China
  2. 2.Faculty of Life ScienceThe University of ManchesterManchesterUK
  3. 3.Achucarro Center for NeuroscienceIKERBASQUE, Basque Foundation for ScienceBilbaoSpain

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