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Differential effects of amphetamine and GBR-12909 on orolingual motor function in young vs aged F344/BN rats

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Abstract

Rationale

Orolingual motor deficits, such as dysarthria and dysphagia, contribute to increased morbidity and mortality in the elderly. In preclinical studies, we and others have reported age-related decreases in tongue motility in both F344 and F344/BN rats. The fact that these deficits are associated with nigrostriatal dopamine (DA), tissue measures suggest that increasing dopamine function might normalize tongue motility.

Objective

The purpose of the current study was to determine whether two indirect dopamine agonists with locomotor-enhancing effects, d-amphetamine (amphetamine; 1 and 2 mg/kg) and GBR-12909 (5, 10, and 20 mg/kg), can improve tongue motility in aged F344/BN rats.

Methods

Young (6 months) and aged (30 months) F344/BN rats licked water from an isometric force disc so that tongue motility (licks/second) and tongue force could be measured as a function of age and drug dose.

Results

Consistent with our previous studies, tongue force was greater and tongue motility was lower in the aged group. Tongue motility was increased by amphetamine but not by GBR-12909. Amphetamine decreased peak tongue force, primarily in the young group. GBR-12909 did not affect tongue force. GBR-12909 increased the number of licks/session in the young group but not in the aged group, while amphetamine increased this measure in both groups.

Conclusion

These results demonstrate differential effects of these drugs on orolingual motor function and suggest that blocking DA uptake is insufficient to increase tongue motility in aging.

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Acknowledgments

This work was supported by NIH grants AG023549, AG026491, a KUMC Biomedical Research Training grant and the KIDDRC Center grant HD02528.

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Authors and Affiliations

  1. Department of Molecular & Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Blvd., Mailstop 3051, Kansas City, KS, 66160, USA

    Susan Smittkamp, Heather Spalding, Hongyu Zhang & John A. Stanford

  2. Landon Center on Aging, University of Kansas Medical Center, Kansas City, KS, USA

    John A. Stanford

  3. Kansas Intellectual & Developmental Disabilities Research Center, University of Kansas Medical Center, Kansas City, KS, USA

    John A. Stanford

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  1. Susan Smittkamp
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  2. Heather Spalding
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  3. Hongyu Zhang
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  4. John A. Stanford
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Correspondence to John A. Stanford.

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Smittkamp, S., Spalding, H., Zhang, H. et al. Differential effects of amphetamine and GBR-12909 on orolingual motor function in young vs aged F344/BN rats. Psychopharmacology 231, 4695–4701 (2014). https://doi.org/10.1007/s00213-014-3620-4

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  • DOI: https://doi.org/10.1007/s00213-014-3620-4

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