Acute administration of THC impairs spatial but not associative memory function in zebrafish
- 672 Downloads
The present study examined the effect of acute administration of endocannabinoid receptor CB1 ligand ∆-9-tetrahydrocannabinol (THC) on intracellular signalling in the brain and retrieval from two different memory systems in the zebrafish (Danio rerio).
First, fish were treated with THC and changes in the phosphorylation level of mitogen-activated protein (MAP) kinases Akt and Erk in the brain were determined 1 h after drug treatment. Next, animals of a second group learned in a two-alternative choice paradigm to discriminate between two colours, whereas a third group solved a spatial cognition task in an open-field maze by use of an ego-allocentric strategy. After memory acquisition and consolidation, animals were pharmacologically treated using the treatment regime as in the first group and then tested again for memory retrieval.
We found an enhanced Erk but not Akt phosphorylation suggesting that THC treatment specifically activated Erk signalling in the zebrafish telencephalon. While CB1 agonist THC did not affect behavioural performance of animals in the colour discrimination paradigm, spatial memory was significantly impaired. The effect of THC on spatial learning is probably specific, since neither motor activity nor anxiety-related behaviour was influenced by the drug treatment. That indicates a striking influence of the endocannabinoid system (ECS) on spatial cognition in zebrafish.
The results are very coincident with reports on mammals, demonstrating that the ECS is functional highly conserved during vertebrate evolution. We further conclude that the zebrafish provides a promising model organism for ongoing research on the ECS.
KeywordsLearning Memory Fish Endocannabinoid system Telencephalon
The authors thank Athena Andreosso and Stefan Löwe for assisting during establishment of the behavioural setups. We thank Andreas Zimmer for his generous support of the experimental work and for providing equipment used in the histological experiments. Önder Albayram is a member of the DFG cluster of Excellence ImmunoSensation. Part of this work was financed by grants to Andras Bilkei-Gorzo (FOR926, SP2; German Research Council) and Önder Albayram (BONFOR). The experiments followed the guidelines of the animal welfare laws and were approved by the Animal Care and Use Committee of the state North Rhine-Westphalia, Germany.
Conflict of interest
No potential conflicts of interest, financial or otherwise are declared by the authors.
- Avdesh A, Chen M, Martin-Iverson MT, Mondal A, Verdile G, Martins RN (2010) Natural colour preference in the zebrafish (Danio rerio). In: Spink AJ, Grieco F, Krips OE, Loijens LWS, Noldus LPJ, Zimmerman PH (eds) Proceedings of measuring behavior. Noldus, Eindhoven, pp 155–157Google Scholar
- Duran E, Vargas JP, Salas C, Papini MR (2000) Effect of telencephalic ablation on appetitive instrumental learning in the goldfish (Carassius auratus). Psicothema 12:520–524Google Scholar
- Duran E, Ocana FM, Gomez A, Jimenez-Moya F, Broglio C, Rodriguez F, Salas C (2008) Telencephalon ablation impairs goldfish allocentric spatial learning in a “hole-board” task. Acta Neurobiol Exp 68:519–525Google Scholar
- Gellermann LW (1933) Chance orders of alternating stimuli in visual discrimination experiments. J Genet Psychol 42:206–208Google Scholar
- Haller J, Mátyás F, Soproni K, Varga B, Barsy B, Németh B, Mikics E, Freund TF, Hájos N (2007) Correlated species differences in the effects of cannabinoid ligands on anxiety and on GABAergic and glutamatergic synaptic transmission. Eur J Neurosci 25:2445–2456PubMedCrossRefPubMedCentralGoogle Scholar
- Saito K, Watanabe S (2005) Experimental analysis of spatial learning in goldfish. Psychol Rec 55:647–662Google Scholar