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Prenatal corticosterone and adolescent URB597 administration modulate emotionality and CB1 receptor expression in mice

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Abstract

Rationale

The central endocannabinoid system (eCB system) sustains the activity of the hypothalamus–pituitary–adrenal (HPA) axis in mediating individual emotional responses. Deviation in maturational trajectories of these two physiological systems, may persistently adjust individual behavioral phenotype.

Objective

We investigated, in outbred CD1 male mice, whether exposure to prenatal stress may influence short- and long-term emotional and neurochemical responses to a pharmacological stimulation of the eCB system during adolescence.

Methods

To mimic prenatal stress, pregnant mice were supplemented with corticosterone in the drinking water (33.3 mg/l); their adolescent male offspring received daily injections of the fatty acid amide hydrolase inhibitor, URB597 (0.4 mg/kg), in order to enhance eCB signaling. Mice were then tested for: locomotor activity during adolescence and locomotor activity, anxiogenic, and anhedonic profiles in adulthood. We analyzed the expression of CB1 receptors (CB1Rs) in prefrontal cortex, hippocampus, striatum, and cerebellum in adulthood.

Results

Corticosterone administration (PC group) resulted, in adolescence, in a reduction in body weight and locomotion, while in adulthood, in increased anxiety-related behavior and reduced CB1Rs expression in cerebellum. URB597 exposure reduced locomotor activity and increased anhedonia in adulthood. CB1Rs were up-regulated in striatum and hippocampus and down-regulated in the cerebellum. PC-URB597 mice failed to show reductions in locomotion; exhibited increased risk assessment behavior; and showed reduced CB1Rs expression within the prefrontal cortex.

Conclusions

Present results provide support to the hypothesis that precocious manipulations mapping onto the HPA axis and eCB system may persistently adjust individual emotional responses and eCB system plasticity.

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Acknowledgments

This research was supported by “ECB-EMOTION: resilience” from the Dep. of Antidrug Policies c/o Presidency of the Council of Ministres (Italy) to GL and SM and from Instituto de Salud Carlos III (Spain), Redes temáticas de Investigación Cooperativa en salud RD06/0001/1013; GRUPOS UCM-BSCH (GRUPO UCM 951579) to EMM and MPV.

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Authors and Affiliations

  1. Section of Behavioral Neuroscience, Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Rome, Italy

    Chiara Ceci, Simone Macrì & Giovanni Laviola

  2. Department of Physiology (Animal Physiology II), Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Universidad Complutense, Madrid, Spain

    Virginia Mela, Eva M. Marco & Maria-Paz Viveros

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  1. Chiara Ceci
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  2. Virginia Mela
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  3. Simone Macrì
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  4. Eva M. Marco
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  5. Maria-Paz Viveros
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  6. Giovanni Laviola
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Correspondence to Giovanni Laviola.

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Ceci, C., Mela, V., Macrì, S. et al. Prenatal corticosterone and adolescent URB597 administration modulate emotionality and CB1 receptor expression in mice. Psychopharmacology 231, 2131–2144 (2014). https://doi.org/10.1007/s00213-013-3367-3

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