Abstract
Rationale
Traumatic events in early life are associated with an increased risk of psychiatric diseases in adulthood. 5-hydroxytryptamine (5-HT)1A receptors play a pivotal role in the 5-HTergic mechanisms associated with the etiology of stress-related disorders.
Objective
The goal of the present study was to investigate whether juvenile stress influences emotional control via postsynaptic 5-HT1A receptor in the hippocampus and amygdala using contextual fear conditioning test in adult rats.
Methods
The rats were subjected to aversive footshock (FS) during the third week of the postnatal period (3wFS group). During the postadolescent period (10–14 weeks postnatal), experiments were performed.
Results
The systemic administration of the 5-HT1A receptor agonist R-(+)-8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) (0.2 mg/kg, i.p.) attenuated the freezing behavior in the non-FS group, but not in the 3wFS group. The bilateral local injection of 8-OH-DPAT (1 μg/side) into the amygdala decreased the freezing behavior in the non-FS group and the 3wFS group. However, the local injection of 8-OH-DPAT (1 μg/side) into the hippocampus decreased the freezing behavior in the non-FS group, but not in the 3wFS group. In a 5-HT1A receptor binding study, the Bmax of the 3wFS group decreased in the dorsal hippocampus, but not the amygdala in comparison with the non-FS group.
Conclusions
The juvenile stress attenuated the hippocampal postsynaptic 5-HT1A receptor function in context-dependent conditioned fear.
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Acknowledgements
The authors thank Dr. Shigeo Miyata and Dr. Katsuzi Suzuki for helpful advice. The authors also thank Dr. Kasane Higuchi and Dr. Kotaro Konno for their excellent technical support. This study was supported by Japanese Ministry of Education grants No. 0677074 (T. I.).
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Matsuzaki, H., Izumi, T., Horinouchi, T. et al. Juvenile stress attenuates the dorsal hippocampal postsynaptic 5-HT1A receptor function in adult rats. Psychopharmacology 214, 329–337 (2011). https://doi.org/10.1007/s00213-010-1987-4
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DOI: https://doi.org/10.1007/s00213-010-1987-4