Psychopharmacology

, Volume 210, Issue 4, pp 585–590 | Cite as

Ginkgo biloba extract treatment increases noncontact erections and central dopamine levels in rats: role of the bed nucleus of the stria terminalis and the medial preoptic area

  • Kuei-Ying Yeh
  • Yan-Zhen Liu
  • Mei-Yun Tai
  • Yuan-Feen Tsai
Original Investigation

Abstract

Rationale

Penile erection is necessary for successful copulation in males. The extract of Ginkgo biloba leaves (EGb 761) significantly facilitates copulation in male rats, but the effect of EGb 761 on noncontact erection (NCE) remains unknown.

Objective

The present study was conducted to evaluate the influence of EGb 761 on NCE in male rats.

Materials and methods

Adult Long-Evans male rats were treated with 50 mg/kg of EGb 761 (experimental group) or distilled water (control group) by gavage for 14 days. The NCE test was carried out after 14 days of EGb 761 treatment, and the latency and the numbers of NCE were recorded. Approximately 14 h following the NCE behavioral tests, animals were sacrificed by means of decapitation, and levels of dopamine in the bed nucleus of the stria terminalis (BNST) and medial preoptic area (MPOA) were measured by means of high-pressure liquid chromatography with electrochemical detection.

Results

Chronic treatment with EGb 761 significantly decreased the NCE latency, but increased the number of NCEs and the dopamine levels in the BNST and MPOA in rats compared to the controls.

Conclusion

Treatment with EGb 761 increased both NCEs and the dopamine contents in the BNST and the MPOA. These results suggest that enhanced NCEs in the rats administered with EGb 761 may be related to dopaminergic activity in the BNST and MPOA.

Keywords

Noncontact erection Dopamine Bed nucleus of the stria terminalis Medial preoptic area EGb 761 

Notes

Acknowledgment

This study was supported by a grant from the National Science Council (NSC 95-2413-H002-006), ROC.

References

  1. Adachi H, Sato Y, Kato R, Hisasue S, Suzuki K, Masumori N, Itoh N, Tsukamoto T (2003) Direct evidence of facilitative actions of dopamine in the medial preoptic area on reflexive and noncontact erections in male rats. J Urol 169:386–389CrossRefPubMedGoogle Scholar
  2. Canteras NS, Simerly RB, Swanson LW (1992) Connections of the posterior nucleus of the amygdala. J Comp Neurol 324:143–179CrossRefPubMedGoogle Scholar
  3. Chen JC, Tsai HW, Yeh KY, Tai MY, Tsai YF (2007) Male sexual behavior and catecholamine levels in the medial preoptic area and arcuate nucleus in middle-aged rats. Brain Res 1184:186–192CrossRefPubMedGoogle Scholar
  4. Chen JC, Tsai HW, Yeh KY, Tai MY, Tsai YF (2008) Correlation of catecholamine levels in the bed nucleus of the stria terminalis and reduced sexual behavior in middle-aged male rats. J Gerontol A Biol Sci Med Sci 63A:678–682Google Scholar
  5. Cohen AJ, Bartlik B (1998) Ginkgo biloba for antidepressant-induced sexual dysfunction. J Sex Marital Ther 24:139–143CrossRefPubMedGoogle Scholar
  6. Dominguez JM, Hull EM (2005) Dopamine, the medial preoptic area, and male sexual behavior. Physiol Behav 86:356–368CrossRefPubMedGoogle Scholar
  7. Emery DE, Sachs BD (1976) Copulatory behavior in male rats with lesions in the bed nucleus of the stria terminalis. Physiol Behav 17:803–806CrossRefPubMedGoogle Scholar
  8. Fallon JH, Moore RY (1978) Catecholamine innervation of the basal forebrain. IV. Topography of the dopamine projection to the basal forebrain and neostriatum. J Comp Neurol 180:545–580CrossRefPubMedGoogle Scholar
  9. Gauthaman K, Adaikan PG (2005) Effect of Tribulus terrestris on nicotinamide adenine dinucleotide phosphate-diaphorase activity and androgen receptors in rat brain. J Ethnopharmacol 96:127–132CrossRefPubMedGoogle Scholar
  10. Gauthaman K, Ganesan AP, Prasad RN (2003) Sexual effects of puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation using a rat model. J Altern Complement Med 9:257–265CrossRefPubMedGoogle Scholar
  11. Hull EM, Wood RI, McKenna KE (2006) The neurobiology of male sexual behavior. In: Neill JD, Pfaff DW (eds) The physiology of reproduction. Elsevier Press, New York, pp 1729–1824Google Scholar
  12. Kim C, Choi H, Kim CC, Kim JK, Kim MS (1976) Influence of ginseng on mating behavior of male rats. Am J Chin Med 4:163–168CrossRefPubMedGoogle Scholar
  13. Knoll J (1997) Sexual performance and longevity. Exp Gerontol 32:539–552CrossRefPubMedGoogle Scholar
  14. Kondo Y, Tomihara K, Sakuma Y (1999) Sensory requirements for noncontact penile erection in the rat. Behav Neurosci 113:1062–1070CrossRefPubMedGoogle Scholar
  15. Krettek JE, Price JL (1978) Amygdaloid projections to subcortical structures within the basal forebrain and brainstem in the rat and cat. J Comp Neurol 178:225–254CrossRefPubMedGoogle Scholar
  16. Liu YC, Salamone JD, Sachs BD (1997) Lesions in medial preoptic area and bed nucleus of stria terminalis: differential effects on copulatory behavior and noncontact erection in male rats. J Neurosci 17:5245–5253PubMedGoogle Scholar
  17. Louilot A, Gonzalez-Mora JL, Guadalupe T, Mas M (1991) Sex-related olfactory stimuli induce a selective increase in dopamine release in the nucleus accumbens of male rats. A voltammetric study. Brain Res 553:313–317CrossRefPubMedGoogle Scholar
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  19. Murphy LL, Cadena RS, Chavez D, Ferraro JS (1998) Effect of American ginseng (Panax quinquefolium) on male copulatory behavior in the rat. Physiol Behav 64:445–450CrossRefPubMedGoogle Scholar
  20. Powers JB, Newman SW, Bergondy ML (1987) MPOA and BNST lesions in male Syrian hamsters: differential effects on copulatory and chemoinvestigatory behaviors. Behav Brain Res 23:181–195CrossRefPubMedGoogle Scholar
  21. Sachs BD (1995) Placing erection in context: the reflexogenic–psychogenic dichotomy reconsidered. Neurosci Biobehav Rev 19:211–224CrossRefPubMedGoogle Scholar
  22. Sachs BD (1997) Erection evoked in male rats by airborne scent from estrous females. Physiol Behav 62:921–924CrossRefPubMedGoogle Scholar
  23. Sachs BD, Akasofu K, Citron JH, Daniels SB, Natoli JH (1994) Noncontact stimulation from estrous females evokes penile erection in rats. Physiol Behav 55:1073–1079CrossRefPubMedGoogle Scholar
  24. Schulz KM, Richardson HN, Romeo RD, Morris JA, Lookingland KJ, Sisk CL (2003) Medial preoptic area dopaminergic responses to female pheromones develop during puberty in the male Syrian hamster. Brain Res 988:139–145CrossRefPubMedGoogle Scholar
  25. Sloley BD, Urichuk LJ, Morley P, Durkin J, Shan JJ, Pang PK, Coutts RT (2000) Identification of kaempferol as a monoamine oxidase inhibitor and potential neuroprotectant in extracts of Ginkgo biloba leaves. J Pharm Pharmacol 52:451–459CrossRefPubMedGoogle Scholar
  26. Tsai HW, Shui HA, Liu HS, Tai MY, Tsai YF (2006) Monoamine levels in the nucleus accumbens correlate with male sexual behavior in middle-aged rats. Pharm Biochem Behav 83:265–270CrossRefGoogle Scholar
  27. Valcourt RJ, Sachs BD (1979) Penile reflexes and copulatory behavior in male rats following lesions in the bed nucleus of the stria terminalis. Brain Res Bull 4:131–133CrossRefPubMedGoogle Scholar
  28. Yeh KY, Pu HF, Kaphle K, Lin SF, Wu LS, Lin JH, Tsai YF (2008) Ginkgo biloba extract enhances male copulatory behavior and reduces serum prolactin levels in rats. Horm Behav 53:225–231CrossRefPubMedGoogle Scholar
  29. Yen TT, Dallo J, Knoll J (1982) The aphrodisiac effect of low doses of (−) deprenyl in male rats. Pol J Pharmacol Pharm 34:303–308PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Kuei-Ying Yeh
    • 1
  • Yan-Zhen Liu
    • 2
  • Mei-Yun Tai
    • 2
  • Yuan-Feen Tsai
    • 2
  1. 1.Department of Physical TherapyHungKuang UniversityTaichung CountyRepublic of China
  2. 2.Department of Physiology, College of MedicineNational Taiwan UniversityTaipei (100)Republic of China

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