Abstract
Rationale
In the absence of agonists and antagonists evidencing appropriate selectivities, individual and interactive properties of D1 and D3 dopamine receptors would be illuminated most powerfully by their co-deletion.
Objectives
To define and contrast the behavioural phenotype of D1/D3 double knockout mice in comparison with wild types, and with individual D1 and D3 mutants.
Methods
Behavioural phenotype was characterised using an ethologically based topographical technique.
Results
On comparison with wild types, D1/D3 double mutants were characterised topographically as follows: increases in sniffing and locomotion, which evidenced delayed habituation; reductions in rearing free, rearing seated, grooming, chewing and stillness. Though the D1/D3 double mutant ethogram comprised elements of both single mutant D1 and D3 lines, this phenotype was largely reflective of the D1 mutant component.
Conclusions
Distinct patterns of initial exploratory behaviour and of temporal change over subsequent habituation were evident across the three genotypes, with particular conservation of the D1 phenotype in D1/D3 double mutants. Under the present conditions, there was little systematic evidence for D1:D3 interactions in the regulation of these aspects of behaviour.
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Acknowledgements
The authors thank Sara Fuchs and Domenico Accili for providing the breeding colony of D3 mutants. This work was supported in part by the National Health and Medical Research Council of Australia. J.J.C. was supported by the Australian National Health and Medical Research Council Brain Network into Mental Diseases; J.D. is a Logan Research Fellow of Monash University; J.J.C. and J.L.W. are supported by the Higher Education Authority of Ireland, a Galen Fellowship from the Irish Brain Research Foundation, and the Research Committee of the Royal College of Surgeons in Ireland.
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Wong, J.Y.F., Clifford, J.J., Massalas, J.S. et al. Essential conservation of D1 mutant phenotype at the level of individual topographies of behaviour in mice lacking both D1 and D3 dopamine receptors. Psychopharmacology 167, 167–173 (2003). https://doi.org/10.1007/s00213-003-1415-0
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DOI: https://doi.org/10.1007/s00213-003-1415-0