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The Lawson-loaded β-cyclodextrin nanocarriers (LB-NCs) a novel targeted cancer cell in stomach and breast cancer as a drug delivery system

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Abstract

Applying nanotechnology to design drug delivery systems is a promising turning point in cancer treatment strategies. In the current study, Lawson, a nonpolar anticancer phytochemical, was entrapped into β-cyclodextrin polymer to evaluate its selective cytotoxicity in several types of human cancer cell lines including MCF-7, AGS, A549, and PC3. The Lawson-loaded β-cyclodextrin nanocarriers (LB-NCs) were produced by applying a high-energy ultrasound-mediated homogenization technique. The LB-NCs were characterized by applying dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), zeta potential, and field emission scanning electron microscopy (FESEM) analysis. Also, the selective cytotoxic impact of the LB-NCs was studied by conducting the MTT assay on human MCF-7, AGS, A549, and PC3 cancer cell lines. Finally, the type of cellular death was evaluated by measuring the cell cycle status and apoptotic gene expression profile of the treated MCF-7 cells by conducting flow cytometry and Q-PCR methods, respectively. The synthesized negatively charged (− 23.8 mV) nanoparticles (348.12 nm) exhibited apoptotic activity in the human breast MCF-7 cancer cells by upregulating the apoptotic gene expression profile (Caspase 3, 8, and 9). The LB-NCs exhibited a significant selective cytotoxic effect on the human cancer cell lines compared with the normal HUVEC cells. However, variable toxic intensities were detected depending on the cancer cell type. Selective cancer cell-depended anticancer activity of the produced LB-NCs has the potential to be considered their safe efficient targeted anticancer activity. However, studying the animal cancer models has to be conducted to verify their selective toxicity and clarify the cellular death mechanism.

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Acknowledgements

This work was supported by the Islamic Azad University, Tehran, Iran, and therefore is appreciated by the author.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ali Kadhim Alwan Alboabdullah

  2. Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran

    Mohammad Taghi Goodarzi

  3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Masoud Homayouni Tabrizi

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  1. Ali Kadhim Alwan Alboabdullah
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  2. Mohammad Taghi Goodarzi
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Ali Kadhim Alwan Alboabdullah: Methodology, Investigation and Writing-Original draft. Mohammad Taghi Goodarzi and Masoud Homayouni Tabrizi: Supervision, Data curation, Conceptualization, Validation and Writing- Reviewing and Editing. The authors declare that all data were generated in-house and that no paper mill was used.

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Correspondence to Masoud Homayouni Tabrizi.

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Alboabdullah, A.K.A., Goodarzi, M.T. & Homayouni Tabrizi, M. The Lawson-loaded β-cyclodextrin nanocarriers (LB-NCs) a novel targeted cancer cell in stomach and breast cancer as a drug delivery system. Naunyn-Schmiedeberg's Arch Pharmacol (2024). https://doi.org/10.1007/s00210-024-03042-6

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