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Study on the mechanism of vitamin E alleviating non-alcoholic fatty liver function based on non-targeted metabolomics analysis in rats

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Abstract

Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome. Vitamin E (VE) has antioxidant properties and can mediate lipid metabolism. Non-targeted metabolomics technology was employed to uncover comprehensively the metabolome of VE in NAFLD rats. NAFLD model was created with a high-fat and high-cholesterol diet (HFD) in rats. NAFLD rats in the VE group were given 75 mg/(kg day) VE. The metabolites in the serum of rats were identified via UPLC and Q-TOF/MS analysis. KEGG was applied for the pathway enrichment. VE improved the liver function, lipid metabolism, and oxidative stress in NAFLD rats induced by HFD. Based on the metabolite profile data, 132 differential metabolites were identified between VE group and the HFD group, mainly including pyridoxamine, betaine, and bretylium. According to the KEGG results, biosynthesis of cofactors was a key metabolic pathway of VE in NAFLD rats. VE can alleviate NAFLD induced by HFD, and the underlying mechanism is associated with the biosynthesis of cofactors, mainly including pyridoxine and betaine.

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Data availability

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Funding

This project was supported by Hangzhou Special Fund for supporting science and technology in the development of biomedical and health industries (grant numbers: 2021WJCY127 and 2021WJCY143), Project of medical and health technology development program in Shandong province (grant numbers: 2019WS366) and PhD Research Foundation of Affiliated Hospital of Jining Medical University (grant numbers: 2017-BS-007).

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Author notes

  1. Baiyun Zhao and Jing Zhang contributed equally to this work.

Authors and Affiliations

  1. Drug Clinical Trial Institution, Hangzhou Normal University Affiliated Hospital, Hangzhou, China

    Baiyun Zhao, Ling Zeng & Chaoxuan Wang

  2. Department of Gastroenterology, Affiliated Hospital of Jining Medical University, Jining, China

    Jing Zhang

  3. Medical Department, Hangzhou Normal University Affiliated Hospital, Hangzhou, China

    Kaiyue Zhao & Wenbin Zhao

  4. Department of Translational Medicine Center, Hangzhou Normal University Affiliated Hospital, 126 Wenzhou Road, Gongshu District, Hangzhou, 310015, Zhejiang, China

    Yajuan Shi & Junping Shi

  5. Department of Clinical Medicine, Hangzhou Normal University, Hangzhou, China

    Jing Liu

  6. Traditional Chinese Pharmacy, China Pharmaceutical University School, Nanjing, China

    Xin Zeng

Authors
  1. Baiyun Zhao
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  2. Jing Zhang
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  3. Kaiyue Zhao
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  4. Wenbin Zhao
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  5. Yajuan Shi
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  7. Ling Zeng
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  8. Chaoxuan Wang
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Contributions

BYZ and JZ carried out the concepts, design and definition of intellectual content, KYZ, WBZ, YJS, JL, LZ, CXW and XZ provided assistance for data acquisition, data analysis and statistical analysis. All authors performed the experiment. The first draft of the manuscript was written by BYZ. JPS revised the manuscript critically for important intellectual content. All authors have read and approved the content of the manuscript, including the authorship list. The authors declare that all data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Junping Shi.

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Ethical approval

All animal care procedures were proceeded in keeping with protocols approved by the Hangzhou Normal University Institutional Committee on Animal Care and Use (HSD20220203). All experiments complied with the Guide for the Care and Use of Laboratory Animals and the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

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The authors declare no competing interests.

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Zhao, B., Zhang, J., Zhao, K. et al. Study on the mechanism of vitamin E alleviating non-alcoholic fatty liver function based on non-targeted metabolomics analysis in rats. Naunyn-Schmiedeberg's Arch Pharmacol 397, 4299–4307 (2024). https://doi.org/10.1007/s00210-023-02864-0

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