Abstract
Doxorubicin (DOX) is a potent and broad-spectrum drug widely used in the treatment of cancer. However, the toxicity and side effects of DOX on various organs limit its clinical use. Approaches using natural antioxidants with these drugs have the potential to alleviate negative side effects. The aim of this study was to investigate the potential protective effect of tannic acid, a polyphenolic compound found naturally in plants, against DOX-induced spleen toxicity. Expression levels of Alox5, Inos, IL-6, Tnf-α, Casp-3, Bax, SOD, GST, CAT and GPx genes were determined using cDNAs obtained from spleen tissues of rats treated with DOX, tannic acid and both. In addition, SOD, CAT, GPx and GST enzyme activities, and GSH and MDA levels were measured in tissues. In the spleen tissues, DOX caused a decrease in the level of GSH and an increase in the level of MDA. In addition, it was determined that DOX had a suppressive effect on CAT, GST, SOD and GPx mRNA levels and its enzyme activities, which are antioxidant system components. The mRNA expression levels of proinflammatory cytokine markers, apoptotic genes, and some factors involved in cell metabolism showed a change compared to the control after DOX application. However, as a result of tannic acid treatment with DOX, these changes approached the values of the control group. The findings showed that tannic acid had a protective effect on the changes in the oxidative stress and inflammation system in the rat spleen as a result of the application of tannic acid together with DOX.
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Authors would like to acknowledge the funding from Atatürk University by Scientific Research Project Coordination Unit (Grant Number: FYL-2021-9395).
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This study was funded by Ataturk University Scientific Research Project Coordination Unit (Grant Number: FYL-2021-9395).
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M.K. and H.C. designed the experiments. M.K. and D.K performed the experiments and wrote the paper. M.K. and H.C. analyzed the data. M.K. was responsible for funding acquisition and project management. The authors declare that all data were generated in-house and that no paper mill was used.
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Kizir, D., Karaman, M. & Ceylan, H. Tannic acid may ameliorate doxorubicin-induced changes in oxidative stress parameters in rat spleen. Naunyn-Schmiedeberg's Arch Pharmacol 396, 3605–3613 (2023). https://doi.org/10.1007/s00210-023-02563-w
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DOI: https://doi.org/10.1007/s00210-023-02563-w