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Development and evaluation of Resveratrol-loaded liposomes in hydrogel-based wound dressing for diabetic foot ulcer

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Abstract

Diabetic foot wounds (DFUs) are counted as one of the most common microvascular complications associated with poorly controlled and chronic diabetes mellitus. It confers a serious challenge to clinical practice, surmounting hyperglycemia-induced disturbance in angiogenesis and endothelial dysfunction, with limited fruitful intervention to control the manifestations of DFUs. Resveratrol (RV) can improve endothelial function and has strong pro-angiogenic properties for the treatment of diabetic foot wounds. The present study aims to design an RV-loaded liposome-in-hydrogel system to effectively heal diabetic foot ulcers. A thin-film hydration method was used to prepare RV-loaded liposomes. Liposomal vesicles were assessed, for various characteristics such as particle size, zeta potential, and entrapment efficiency. The best-prepared liposomal vesicle was then incorporated into 1% carbopol 940 gel to develop a hydrogel system. The RV-loaded liposomal gel showed improved skin penetration. To assess the efficacy of the developed formulation, a diabetic foot ulcer animal model was used. The topical application of the developed formulation significantly reduced blood glucose and increased glycosaminoglycans (GAGs) to improve ulcer healing as well as wound closure on day 9. Faster re-epithelization, proliferation of fibroblast, formation of collagen, and reduced inflammatory cell infiltration at the wound site were also noted. Results indicate that RV-loaded liposomes in hydrogel-based wound dressing significantly accelerate wound healing in diabetic foot ulcers by restoring the altered wound healing process in diabetics.

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Acknowledgements

The authors are thankful to the Faculty of Pharmacy, Integral University, for providing all necessary facilities related to the present work.

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Authors and Affiliations

  1. Faculty of Pharmacy, Integral University, Dasauli-Kursi Road, Lucknow, 226026, India

    Supriya Pandey, Arshiya Shamim, Mohammad Shaif & Poonam Kushwaha

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  1. Supriya Pandey
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  2. Arshiya Shamim
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  3. Mohammad Shaif
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  4. Poonam Kushwaha
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Contributions

PK make a substantial contribution to conception and design. SP and AS participated in the analysis and interpretation of data. SP and MS carried out the acquisition of data and wrote the paper with input from all authors. All authors read and approved the final manuscript.

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Correspondence to Poonam Kushwaha.

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Ethics approval

The study was approved by the Faculty of Pharmacy, Integral University, Lucknow, India (CPCSEA approval#-IU/IAEC/19/17). All animal experiments were performed strictly in humane as possible and complied with the guidelines for animal care with the approval of the Institutional Animal Ethical Committee (IAEC).

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Pandey, S., Shamim, A., Shaif, M. et al. Development and evaluation of Resveratrol-loaded liposomes in hydrogel-based wound dressing for diabetic foot ulcer. Naunyn-Schmiedeberg's Arch Pharmacol 396, 1811–1825 (2023). https://doi.org/10.1007/s00210-023-02441-5

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