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Astringin protects LPS-induced toxicity by suppressing oxidative stress and inflammation via suppression of PI3K/AKT/NF-κB pathway for pediatric acute lung injury

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Abstract

Acute lung injury (ALI) is a major pathophysiological problem defined by severe inflammation and acute disease with substantial morbidity and death. It is known that lipopolysaccharide (LPS) induces ALI by causing oxidative stress and inflammation. The goal of this study was to investigate the protective effect of astringin on LPS-induced ALI and probable pathways. Astringin is a stilbenoid, the 3-β-D-glucoside of piceatannol, mainly found in the bark of Picea sitchensis. The findings showed that astringin prevented LPS-induced cellular damage by reducing the generation of oxidative stress in LPS-stimulated A549 lung epithelial cells. Furthermore, astringin extensively decreased the production of inflammatory factors such as TNF-α, IL-1β, and IL-6. In addition, the western blot results revealed that the ability of astringin to reduce oxidative stress and the generation of inflammatory cytokines by inhibiting the ROS-mediated PI3K/AKT/NF-κB pathway could be the reason for its protective effect against LPS-induced ALI. Overall, the results suggest that astringin could be a possible inhibitor of ALI triggered by LPS for pediatric lung injury.

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Authors and Affiliations

  1. Department of Neonatology, The First People’s Hospital of Wenling, No.333, Chuanan South Road, Chengxi Street, Wenling, 317500, Zhejiang, China

    Ling Wang, Shanshan Jiang, Xiaoxiao Li, Tingting Lin & Tao Qin

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  1. Ling Wang
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  2. Shanshan Jiang
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  3. Xiaoxiao Li
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  4. Tingting Lin
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  5. Tao Qin
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Contributions

LW: conceptualization, performed the experiments, data analysis, and writing—original draft. SJ: performed the experiments, data analysis, and writing—original draft. XL: performed the experiments, data analysis, and writing—original draft. TL: Conceptualization, supervision, data analysis, and reviewing—original draft. TQ: conceptualization, supervision, data analysis, and reviewing—original draft. All authors contributed to the article and approved the submitted version. The authors declare that all data were generated in-house and that no paper mill was used.

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Correspondence to Tingting Lin or Tao Qin.

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Wang, L., Jiang, S., Li, X. et al. Astringin protects LPS-induced toxicity by suppressing oxidative stress and inflammation via suppression of PI3K/AKT/NF-κB pathway for pediatric acute lung injury. Naunyn-Schmiedeberg's Arch Pharmacol 396, 2369–2377 (2023). https://doi.org/10.1007/s00210-023-02439-z

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