Abstract
Peptic ulcers are lesions in the gastric and duodenal mucosa generated by an imbalance between protective factors (gastroduodenal mucus secretion, bicarbonate production, adequate blood flow) and harmful factors (excess pepsin or hydrochloric acid). Some drugs used in peptic ulcer therapy are associated with adverse effects. The aim of this study was to evaluate the antiulcerogenic and healing activity of hecogenin acetate (HA) in acute and chronic models of gastric lesions in rodents. The antiulcerogenic activity of HA was evaluated in models of gastric lesions induced by absolute ethanol and in acidified ethanol with HA (5, 10, and 20 mg/kg). For the model of gastric lesions induced by ischemia and reperfusion, rats were pre-treated with HA (5, 10, 20 mg/kg). After that, they were submitted to 30 min of ischemia, followed by 1 h of reperfusion. To evaluate the healing activity was induced gastric ulcer using acetic acid (80%) in rats. After 24 h, they were treated for 7 consecutive days with HA (10 and 20 mg/kg). They were evaluated the possible signs of toxicity, measurement of the lesions, collagen deposition, and histological analysis. HA significantly reduced the area of the lesion in models of gastric lesions induced by absolute and acidified ethanol, ischemia-induced gastric lesions and reperfusion, and regarding healing. In the collagen deposition, the presence and increase of collagen demonstrate the healing effect. The AH has antiulcerogenic and healing potential demonstrated by the decrease in gastric injury and presence of collagen fibers, respectively.
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This work was supported by the Coordination of Improvement of Higher Education Personnel (CAPES) and Research Support Foundation of the State of Piauí (FAPEPI).
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AJCS, FVS, AFSCV, and RCMO conceived and designed research. AJCS. BPSN, DSC, MCS, CESC, FVS, JAN, and AFSCV conducted experiments. JSSQ and LJQJ contributed new reagents or analytical tools. AJCS, DSC, PHMN, and RCMO analyzed data and prepared figures. AJCS and RCMO wrote the manuscript. All authors reviewed and approved the manuscript.
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This study was performed in line with the principles of ARRIVE Guidelines for animal research, and approval was granted by the Ethics Committee for Animal Use of Federal University of Piauí – CEUA/UFPI (2018/516).
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Sousa, A.J.C., de Sousa Neto, B.P., da Costa, D.S. et al. Antiulcerogenic and healing activity of hecogenin acetate in rodents. Naunyn-Schmiedeberg's Arch Pharmacol 396, 759–769 (2023). https://doi.org/10.1007/s00210-022-02341-0
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DOI: https://doi.org/10.1007/s00210-022-02341-0