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Sodium acetate ameliorated systemic and renal oxidative stress in high-fructose insulin-resistant pregnant Wistar rats

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Abstract

Pregnancy is an insulin-resistant condition especially at near term predisposing maternal kidneys to hyperinsulinemia-induced oxidative stress. The impact of fructose on renal metabolic dysregulation and oxidative stress in pregnancy requires elucidation. Short-chain fatty acids (SCFAs) are known for protective roles in oxidative stress conditions. Therefore, the study aimed at investigating fructose-induced glucose dysregulation and renal oxidative stress in pregnant and non-pregnant rats and the possible preventive role of SCFA, acetate. Thirty female Wistar rats were grouped (n = 5/group). Three groups were made pregnant (P); the other three remained non-pregnant (NP). Both pregnant and non-pregnant rats received drinking water (control), 10% fructose (w/v) (NP+F or P+F), and 10% (w/v) fructose plus sodium acetate (200 mg/kg) (NP+F+A or P+F+A) for 3 weeks. Renal and plasma glutathione antioxidant index (GSH/GSSG), G6PDH, and adenosine were significantly lower in NP+F and P+F groups compared with control while renal and plasma adenosine deaminase (ADA), xanthine oxidase (XO), uric acid (UA), lactate dehydrogenase (LDH), and malonaldehyde (MDA) were significantly elevated in NP+F and P+F groups compared with controls. HOMA-IR showed marked impairment in both NP+F and P+F groups. The P+F group revealed greater suppression in plasma and renal G6PDH-dependent antioxidant index, adenosine, and aggravation of LDH, MDA compared with the NP+F group (p < 0.05). Sodium acetate reduces plasma and renal surrogate oxidative stress markers, improved G6PD-dependent antioxidant index, and HOMA-IR in NP+F and P+F groups. Pregnancy exacerbates fructose-induced insulin resistance and renal oxidative stress whereas acetate ameliorated fructose-induced redox and glucose dysregulation in pregnant and non-pregnant rats.

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Data for the research work were made available at submission.

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Acknowledgements

The authors acknowledge the technical support of HOPE Cardiometabolic Research Team and Mr. Adebowale of Bridge Biotech is also appreciated for the laboratory analysis.

Funding

There was no external source of funding for the work.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, P.M.B. 1515, Ilorin, 240001, Nigeria

    Adewumi Oluwafemi Oyabambi, Emmanuel Damilare Areola, Salam Babatunde Saliu & Lawrence Aderemi Olatunji

  2. Cardiometabolic, Microbiome and Applied Physiology Laboratory, Physiology Program, College of Health Sciences, Bowen University, Iwo, Nigeria

    Olugbenga Samuel Michael

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  1. Adewumi Oluwafemi Oyabambi
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  2. Olugbenga Samuel Michael
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  4. Salam Babatunde Saliu
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  5. Lawrence Aderemi Olatunji
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Contributions

AOO, OSM, and LAO conceived and designed the research. AOO, EDA, and SBS conducted the experiments. AOO, OSM, EDA, SBS, and LAO contributed to the new reagents and analytical kits. AOO, OSM, EDA, and LAO analyzed and interpreted the data. AOO, OSM, EDA, SBS, and LAO drafted the manuscript. AOO, OSM, EDA, SBS, and LAO. All authors read and approved the manuscript, and all data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Adewumi Oluwafemi Oyabambi.

Ethics declarations

Ethics approval and consent to participate

Ethical approval for the work was obtained from the University of Ilorin Ethical Review Committee with protocol number UERC/ASN/2016/357, having passed the Faculty of Basic Medical Sciences, University of Ilorin Ethical Review Committee. The investigation was conducted in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and was approved by the Institutional Review Board of the University of Ilorin, with protocol identification number UERC/ASN/2016/357, and every effort was made to minimize both the number of animals used and their sufferings.

The following underlisted authors agreed and freely participated in the review of this work and made a sizeable contribution to the progress of the work.

Adewumi Oluwafemi Oyabambi, oyabambi.ao@unilorin.edu.ng

Olugbenga Samuel Micheal, michaelolugbenga2@gmail.com

Emmanuel Damilare Areola, areola577@gmail.com

Salam Babatunde Saliu, salambat50@gmail.com

Lawrence Aderemi Olatunji, tunjilaw@unilorin.edu.ng

Consent for publication

The following underlisted authors agreed that the manuscript titled “Sodium acetate ameliorated systemic and renal oxidative stress in high-fructose insulin-resistant pregnant Wistar rats.” be published within your Journal, Naunyn-Schmiedeberg's Archives of Pharmacology.

Adewumi Oluwafemi Oyabambi, oyabambi.ao@unilorin.edu.ng

Olugbenga Samuel Micheal, michaelolugbenga2@gmail.com

Emmanuel Damilare Areola, areola577@gmail.com

Salam Babatunde Saliu, salambat50@gmail.com

Lawrence Aderemi Olatunji, tunjilaw@unilorin.edu.ng

Conflict of interest

The authors declare no competing interests.

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Oyabambi, A.O., Michael, O.S., Areola, E.D. et al. Sodium acetate ameliorated systemic and renal oxidative stress in high-fructose insulin-resistant pregnant Wistar rats. Naunyn-Schmiedeberg's Arch Pharmacol 394, 1425–1435 (2021). https://doi.org/10.1007/s00210-021-02058-6

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  • DOI: https://doi.org/10.1007/s00210-021-02058-6

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