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Calcium dobesilate prevents cisplatin-induced nephrotoxicity by modulating oxidative and histopathological changes in mice

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Abstract

Cisplatin is one of the synthetic cancer medicines with nephrotoxicity being one of its major side effects. Past research shows that calcium dobesilate (CaD), as a vascular protective agent in diabetic retinopathy, has antioxidant properties. Thus, this study aims to evaluate the protective effects of CaD in cisplatin-induced nephrotoxicity in mice. A many as 28 mice, in the present experimental research, were randomly distributed into four groups, including control, cisplatin (the intraperitoneal administration of 20 mg/kg cisplatin only on the first day of the experiment), cisplatin + CaD 50 (cisplatin with the oral administration of 50 mg/kg CaD), and cisplatin + CaD 100 (cisplatin with the oral administration of 100 mg/kg CaD). The treated groups received CaD by oral gavage for 4 constitutive days. On the fifth day, the mice were sacrificed, and some biochemical (serum levels of Cr and BUN, renal tissue levels of MDA, and renal activities of SOD and GPx) and pathological parameters were evaluated. Based on the results, there was a significant decrease in the renal SOD and GPx activities; in contrast, there was a significant increase in the BUN, Cr, and renal MDA levels following administering cisplatin. However, the CaD treatment (100 mg/kg) significantly attenuated these alterations. In addition, the kidney’s histological examination of kidneys confirmed the nephroprotective effects of CaD. The findings proved the protective impact of CaD on cisplatin-induced nephrotoxicity by an improvement in the oxidative stress factors.

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Acknowledgments

The authors would like to extend their thanks to the clinical research development unit in Ali-Ibn Abi-Talib Hospital, Rafsanjan University of Medical Sciences, for its support and collaboration.

Funding

This article is based on Dr. Ali Fathinejad’s thesis for obtaining a degree in general medicine from Rafsanjan University of Medical Sciences with Grant Number 97062.

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Authors and Affiliations

  1. Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Gholamreza Bazmandegan & Ayat Kaeidi

  2. Department of Family Medicine, Ali-Ibn Abi-Talib Hospital, School of medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Gholamreza Bazmandegan

  3. Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

    Iman Fatemi

  4. Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Ayat Kaeidi

  5. Department of Pathology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Morteza Khademalhosseini

  6. Clinical Research Development Unit, Ali-Ibn Abi-Talib Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Morteza Khademalhosseini

  7. Student Research Committee, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Ali Fathinejad & Morteza Amirteimoury

Authors
  1. Gholamreza Bazmandegan
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  2. Iman Fatemi
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  3. Ayat Kaeidi
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  4. Morteza Khademalhosseini
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  5. Ali Fathinejad
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Contributions

Conceived and designed the experiments: GB and MA. Performed the experiments: BG, AK, MK, and AF. Analyzed the data: GB and MA. Contributed reagents/materials/analysis tools: AK, GB, and IF. Wrote the paper: IF and GB. The authors declare that all data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Morteza Amirteimoury.

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All experiments were performed in accordance with the guidelines set by the ethical committee under ethics code IR.RUMS.REC.1397.028.

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The authors declare that they have no conflict of interest.

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Bazmandegan, G., Fatemi, I., Kaeidi, A. et al. Calcium dobesilate prevents cisplatin-induced nephrotoxicity by modulating oxidative and histopathological changes in mice. Naunyn-Schmiedeberg's Arch Pharmacol 394, 515–521 (2021). https://doi.org/10.1007/s00210-020-01990-3

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