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ORY supplementation mitigates acetaminophen-induced acute liver failure in male mice: role of oxidative stress and apoptotic markers

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Abstract

The aim of the present study was to assess the possible protective effect of γ-oryzanol (ORY) supplementation in a model of acute liver failure (ALF) induced by acetaminophen (APAP) in mice. Male Swiss strain mice were supplemented with ORY (10 and 50 mg/kg, per oral route) daily for 7 days. One hour after the last supplementation, animals received APAP (300 mg/kg, intraperitoneal). Twenty-four hours after APAP administration, mice were euthanized, and biochemical and histopathological determinations were performed. Histopathological analysis revealed that APAP caused vascular congestion, loss of cellular structure, and cellular infiltration in hepatocytes. Moreover, it caused oxidative damage (enzymatic and non-enzymatic analysis of oxidative stress), with loss of hepatic function leading to cell apoptosis (apoptotic parameters). ORY supplementation (ORY-10 and ORY-50) protected against all changes in ALF model. Thus, the protective effect of ORY supplementation was due to modulation of antioxidant defenses avoiding the apoptotic process.

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Acknowledgments

Special thanks to C.R.J for making the study possible.

Funding

This study was financially supported by the Federal University of Pampa. This study was financed in part by the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) – Finance Code 001.

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Authors and Affiliations

  1. Laboratory of Pharmacological and Toxicological evaluations applied to Bioactive Molecules (Laftambio Pampa), Federal University of Pampa, Campus Itaqui, Itaqui, RS, 97650-000, Brazil

    Marcelo Gomes de Gomes, Lucian Del Fabbro, André Rossito Goes, Leandro Cattelan Souza, Silvana Peterini Boeira & Cristiano Ricardo Jesse

  2. Department of Pharmacy, University of Contested, Campus Mafra, Mafra, 89306-076, Brazil

    Lucian Del Fabbro

Authors
  1. Marcelo Gomes de Gomes
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  2. Lucian Del Fabbro
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  3. André Rossito Goes
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  4. Leandro Cattelan Souza
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  5. Silvana Peterini Boeira
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  6. Cristiano Ricardo Jesse
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Contributions

M.G.G: Conceptualization and writing. L.D.F., A.R.G., L.C.S., S.P.B, and C.R.J.: experimental methodology. All listed authors have agreed to the final submission of manuscript. In addition, the authors declare that all data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Marcelo Gomes de Gomes.

Ethics declarations

Mice were used according to the National Institutes of Health guide for the care and use of Laboratory animals (NIH Publications No. 8023, revised 1978) and guidelines of the Committee on Care and Use of Experimental Animal Resources of the Federal University of UNIPAMPA, Uruguaiana, Brazil. (Protocol 021/2014).

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The authors declare that they have no conflicts of interest.

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de Gomes, M.G., Del Fabbro, L., Goes, A.R. et al. ORY supplementation mitigates acetaminophen-induced acute liver failure in male mice: role of oxidative stress and apoptotic markers. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2129–2137 (2020). https://doi.org/10.1007/s00210-020-01930-1

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  • DOI: https://doi.org/10.1007/s00210-020-01930-1

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