Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 392, Issue 9, pp 1107–1119 | Cite as

Terminalia arjuna extract and arjunic acid mitigate cobalt chloride–induced hypoxia stress–mediated apoptosis in H9c2 cells

  • T. Mohan Manu
  • T. AnandEmail author
  • M. D. Pandareesh
  • P. Bhuvanesh Kumar
  • Farhath Khanum
Original Article


Arjunic acid (AA) is one of the major active component of Terminalia arjuna known for its health benefits. In the present study, we evaluated cardioprotective potential of Terminalia arjuna extract (TAE) and AA against cobalt chloride (CoCl2)–induced hypoxia damage and apoptosis in rat cardiomyocytes. TAE (50 μg/ml) and AA (8 μg/ml) significantly (p < 0.001) protected H9c2 cells as evidenced by cell viability assays against CoCl2 (1.2 mM)-induced cytotoxicity. TAE and AA pretreatments protected the cells from oxidative damage by decreasing the generation of free radicals (ROS, hydroperoxide, and nitrite levels). TAE and AA pretreatments retained mitochondrial membrane potential by alleviating the rate of lipid peroxidation induced by CoCl2 treatment. TAE and AA pretreatments elevated antioxidant status including phase II antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and total glutathione levels against CoCl2-induced oxidative stress. Further immunoblotting studies confirmed anti-apoptotic effects of TAE and AA by alleviating the phosphorylation of JNK and c-jun and also by regulating protein expression levels of Bcl2, Bax, caspase 3, heat shock protein-70, and inducible nitric oxide synthase. Overall, our results suggest that both the extract and the active component exhibit antioxidant and anti-apoptotic defense against CoCl2-induced hypoxic injury.


Terminalia arjuna Arjunic acid Cobalt chloride Hypoxia Oxidative stress Apoptosis c-Jun N-terminal kinase 



All the authors are grateful to Director DFRL for providing constant support and necessary facilities to conduct the research work. Mohan Manu T is thankful to DST for providing INSPIRE Fellowship.

Authors’ contributions

TA and MMT conceived and designed the research.

MMT and MDP conducted the experiments.

MMT and MDP analyzed the data and written the manuscript.

BKP helped in the extraction of Terminalia arjuna extract.

FK and TA reviewed and corrected the manuscript.

All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • T. Mohan Manu
    • 1
  • T. Anand
    • 1
    Email author
  • M. D. Pandareesh
    • 1
  • P. Bhuvanesh Kumar
    • 1
  • Farhath Khanum
    • 1
  1. 1.Nutrition, Biochemistry and Toxicology DivisionDefence Food Research LaboratoryMysuruIndia

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