Abstract
Nicotine abuse is associated with variety of diseases including arrhythmias, most often atrial fibrillation (AF). Altered inward rectifier potassium currents including acetylcholine-sensitive current I K(Ach) are known to be related to AF pathogenesis. Since relevant data are missing, we aimed to investigate I K(Ach) changes at clinically relevant concentrations of nicotine. Experiments were performed by the whole cell patch clamp technique at 23 ± 1 °C on isolated rat atrial myocytes. Nicotine was applied at following concentrations: 4, 40 and 400 nM; ethanol at 20 mM (∼0.09%). Nicotine at 40 and 400 nM significantly activated constitutively active component of I K(Ach) with the maximum effect at 40 nM (an increase by ∼100%); similar effect was observed at −110 and −50 mV. Changes at 4 nM nicotine were negligible on average. Coapplication of 40 nM nicotine and 20 mM ethanol (which is also known to activate this current) did not show cumulative effect. In the case of acetylcholine-induced component of I K(Ach), a dual effect of nicotine and its correlation with the current magnitude in control were apparent: the current was increased by nicotine in the cells showing small current in control and vice versa. The effect of 40 and 400 nM nicotine on acetylcholine-induced component of I K(Ach) was significantly different at −110 and −50 mV. We conclude that nicotine at clinically relevant concentrations significantly increased constitutively active component of I K(Ach) and showed a dual effect on its acetylcholine-induced component, similarly as ethanol. Synchronous application of nicotine and ethanol did not cause additive effect.
Similar content being viewed by others
References
Amorós I, Dolz-Gaitón P, Gómez R, Matamoros M, Barana A, de la Fuente MG, Núñez M, Pérez-Hernández M, Moraleda I, Gálvez E, Iriepa I, Tamargo J, Caballero R, Delpón E (2013) Propafenone blocks human cardiac Kir2.X channels by decreasing the negative electrostatic charge in the cytoplasmic pore. Biochem Pharmacol 86:267–278. doi:10.1016/j.bcp.2013.04.023
Andrássy G, Szabo A, Dunai A, Simon E, Nagy T, Trummer Z, Tahy A, Varro A (2003) Acute effects of cigarette smoking on the QT interval in healthy smokers. Am J Cardiol 92:489–492. doi:10.1016/S0002-9149(03)00678-7
Arora R, Ng J, Ulphani J, Mylonas I, Subacius H, Shade G, Gordon D, Morris A, He X, Lu Y, Belin R, Goldberger JJ, Kadish AH (2007) Unique autonomic profile of the pulmonary veins and posterior left atrium. J Am Coll Cardiol 49:1340–1348. doi:10.1016/j.jacc.2006.10.075
Aryal P, Dvir H, Choe S, Slesinger PA (2009) A discrete alcohol pocket involved in GIRK channel activation. Nat Neurosci 12:988–995. doi:10.1038/nn.2358
Baden L, Weiss ST, Thomas HE, Sparrow D (1982) Smoking status and the electrocardiogram: a cross-sectional and longitudinal study. Arch Environ Health 37:365–369
Bébarová M, Matejovič P, Pásek M, Šimurdová M, Šimurda J (2005) Effect of ajmaline on transient outward current in rat ventricular myocytes. Gen Physiol Biophys 24:27–45
Bébarová M, Matejovič P, Pásek M, Šimurdová M, Šimurda J (2014) Dual effect of ethanol on inward rectifier potassium current I K1 in rat ventricular myocytes. J Physiol Pharmacol 65:497–509
Bébarová M, Hořáková Z, Kula R (2016a) Addictive drugs, arrhythmias, and cardiac inward rectifiers. See comment in PubMed Commons belowEuropace 2016 Jun 14 (in press). doi:10.1093/europace/euw071
Bébarová M, Matejovič P, Pásek M, Hořáková Z, Hošek J, Šimurdová M, Šimurda J (2016b) Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine. Naunyn Schmiedeberg's Arch Pharmacol 389:1049–1058. doi:10.1007/s00210-016-1265-z
Bodhinathan K, Slesinger PA (2013) Molecular mechanism underlying ethanol activation of G-protein-gated inwardly rectifying potassium channels. Proc Natl Acad Sci U S A 110:18309–18314. doi:10.1073/pnas.1311406110
Caballero R, Dolz-Gaitón P, Gómez R, Amorós I, Barana A, González de la Fuente M, Osuna L, Duarte J, López-Izquierdo A, Moraleda I, Gálvez E, Sánchez-Chapula JA, Tamargo J, Delpón E (2010) Flecainide increases Kir2.1 currents by interacting with cysteine 311, decreasing the polyamine-induced rectification. Proc Natl Acad Sci U S A 107:15631–15636. doi:10.1073/pnas.1004021107
Chatterjee S, Kumar S, Dey SK, Chatterjee P (1989) Chronic effect of smoking on the electrocardiogram. Jpn Heart J 30:827–839
Choragudi NL, Aronow WS, DeLuca AJ (2003) Nicotine gum-induced atrial fibrillation. Heart Dis Hagerstown Md 5:100–101
Dobrev D, Friedrich A, Voigt N, Jost N, Wettwer E, Christ T, Knaut M, Ravens U (2005) The G protein-gated potassium current I(K,ACh) is constitutively active in patients with chronic atrial fibrillation. Circulation 112:3697–3706. doi:10.1161/CIRCULATIONAHA.105.575332
Dolz-Gaitón P, Caballero R, Gómez R, Barana A, Amorós I, Gonzáles de la Fuente M, Tamargo J, Delpón E (2012) Dual effects of propafenone on Kir2.x channels. In: Proceedings of the 6th European Congress of Pharmacology, EPHAR 2012, Granada, Spain
Ehrlich JR (2008) Inward rectifier potassium currents as a target for atrial fibrillation therapy. J Cardiovasc Pharmacol 52:129–135. doi:10.1097/FJC.0b013e31816c4325
Ferrer T, Ponce-Balbuena D, López-Izquierdo A, Aréchiga-Figueroa IA, de Boer TP, van der Heyden MA, Sánchez-Chapula JA (2011) Carvedilol inhibits Kir2.3 channels by interference with PIP2-channel interaction. Eur J Pharmacol 668:72–77. doi:10.1016/j.ejphar.2011.05.067
Fleg JL, Kennedy HL (1982) Cardiac arrhythmias in a healthy elderly population: detection by 24-hour ambulatory electrocardiography. Chest 81:302–307. doi:10.1378/chest.81.3.302
Goldenberg I, Moss AJ, McNitt S, Zareba W, Daubert JP, Hall WJ, Andrews ML, Multicenter Automatic Defibrillator Implantation Trial-II Investigators (2006) Cigarette smoking and the risk of supraventricular and ventricular tachyarrhythmias in high-risk cardiac patients with implantable cardioverter defibrillators. J Cardiovasc Electrophysiol 17:931–936. doi:10.1111/j.1540-8167.2006.00526.x
Gritz ER, Baer-Weiss V, Benowitz NL, Van Vunakis H, Jarvik ME (1981) Plasma nicotine and cotinine concentrations in habitual smokeless tobacco users. Clin Pharmacol Ther 30:201–209. doi:10.1038/clpt.1981.149
Hanna ST, Cao K, Sun X, Wang R (2005) Mediation of the effect of nicotine on Kir6.1 channels by superoxide anion production. J Cardiovasc Pharmacol 45:447–455
Haass M, Kübler W (1997) Nicotine and sympathetic neurotransmission. Cardiovasc Drugs Ther 10:657–665
Heijman J, Voigt N, Nattel S, Dobrev D (2014) Cellular and molecular electrophysiology of atrial fibrillation initiation, maintenance, and progression. Circ Res 114:1483–1499. doi:10.1161/CIRCRESAHA.114.302226
Heijman J, Algalarrondo V, Voigt N, Melka J, Wehrens XHT, Dobrev D, Nattel S (2016) The value of basic research insights into atrial fibrillation mechanisms as a guide to therapeutic innovation: a critical analysis. Cardiovasc Res 109:467–479. doi:10.1093/cvr/cvv275
Henningfield JE, Stapleton JM, Benowitz NL, Grayson RF, London ED (1993) Higher levels of nicotine in arterial than in venous blood after cigarette smoking. Drug Alcohol Depend 33:23–29. doi:10.1016/0376-8716(93)90030-T
Hořáková Z, Matejovič P, Pásek M, Hošek J, Šimurdová M, Šimurda J, Bébarová M (2016) Effect of ethanol and acetaldehyde at clinically relevant concentrations on atrial inward rectifier potassium current IK1: separate and combined effect. J Physiol Pharmacol 67:339–351
Ileri M, Yetkin E, Tandoğan I, Hisar I, Atak R, Senen K, Cehreli S, Demirkan D (2001) Effect of habitual smoking on QT interval duration and dispersion. Am J Cardiol 88:322–325. doi:10.1016/S0002-9149(01)01653-8
İlgenli TF, Tokatlı A, Akpınar O, Kılıçaslan F (2015) The effects of cigarette smoking on the Tp-e interval, Tp-e/QT ratio and Tp-e/QTc ratio. Adv Clin Exp Med 24:973. doi:10.17219/acem/28114
Mills EJ, Thorlund K, Eapen S, Wu P, Prochaska JJ (2014) Cardiovascular events associated with smoking cessation pharmacotherapies: a network meta-analysis. Circulation 129:28–41. doi:10.1161/CIRCULATIONAHA.113.003961
Murail S, Howard RJ, Broemstrup T, Bertaccini EJ, Harris RA, Trudell JR, Lindahl E (2012) Molecular mechanism for the dual alcohol modulation of cys-loop receptors. PLoS Comput Biol 8:e1002710. doi:10.1371/journal.pcbi.1002710 (accessed February 5, 2016)
Nunes JP, Barbosa E, Lopes L, Alves C, Gonçalves FR (2001) Nicotine nasal inhalation, atrial fibrillation and seizures. Cardiology 96:58. doi: 47389
Ondrejka J, Giorgio G (2015) Type 1 Brugada pattern associated with nicotine toxicity. J Emerg Med 49:e183–e186. doi:10.1016/j.jemermed.2015.08.008
Rigotti NA, Eagle KA (1986) Atrial fibrillation while chewing nicotine gum. JAMA 255:1018
Russell MA, Jarvis M, Iyer R, Feyerabend C (1980) Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. Br Med J 280:972–976
Sarmast F, Kolli A, Zaitsev A, Parisian K, Dhamoon AS, Guha PK, Warren M, Anumonwo JM, Taffet SM, Berenfeld O, Jalife J (2003) Cholinergic atrial fibrillation: I(K,ACh) gradients determine unequal left/right atrial frequencies and rotor dynamics. Cardiovasc Res 59:863–873. doi:10.1016/S0008-6363(03)00540-6
Satoh H (1997) Effects of nicotine on spontaneous activity and underlying ionic currents in rabbit sinoatrial nodal cells. Gen Pharmacol 28:39. doi:10.1016/S0306-3623(96)00168-1
Satoh H (2002) Modulation by nicotine of the ionic currents in Guinea pig ventricular cardiomyocytes. Relatively higher sensitivity to IKr and IKl. Vasc Pharmacol 39:55–61. doi:10.1016/S1537-1891(02)00194-5
Stewart PM, Catterall JR (1985) Chronic nicotine ingestion and atrial fibrillation. Br Heart J 54:222–223
Taşolar H, Ballı M, Bayramoğlu A, Otlu YÖ, Cetin M, Altun B, Cakıcı M (2014) Effect of smoking on Tp-e interval, Tp-e/QT and Tp-e/QTc ratios as indices of ventricular arrhythmogenesis. Heart Lung Circ 23:827–832. doi:10.1016/j.hlc.2014.03.016
Teneggi V, Squassante L, Iavarone L, Milleri S, Bye A, Gomeni R (2002) Correlation and predictive performances of saliva and plasma nicotine concentration on tobacco withdrawal-induced craving. Br J Clin Pharmacol 54:407–414. doi:10.1046/j.0306-5251.2002.01650.x
Voigt N, Friedrich A, Bock M, Wettwer E, Christ T, Knaut M, Strasser RH, Ravens U, Dobrev D (2007) Differential hosphorylation-dependent regulation of constitutively active and muscarinic receptor-activated IK,ACh channels in patients with chronic atrial fibrillation. Cardiovasc Res 74:426–437. doi:10.1016/j.cardiores.2007.02.009
Voigt N, Trausch A, Knaut M, Matschke K, Varró A, Van Wagoner DR, Nattel S, Ravens U, Dobrev D (2010) Left-to-right atrial inward rectifier potassium current gradients in patients with paroxysmal versus chronic atrial fibrillation. Circ Arrhythm Electrophysiol 3:472–480. doi:10.1161/CIRCEP.110.954636
Voigt N, Heijman J, Trausch A, Mintert-Jancke E, Pott L, Ravens U, Dobrev D (2013) Impaired Na+-dependent regulation of acetylcholine-activated inward-rectifier K+ current modulates action potential rate dependence in patients with chronic atrial fibrillation. J Mol Cell Cardiol 61:142–152. doi:10.1016/j.yjmcc.2013.03.011
Voigt N, Abu-Taha I, Heijman J, Dobrev D (2014) Constitutive activity of the acetylcholine-activated potassium current IK,ACh in cardiomyocytes. Adv Pharmacol 70:393–409. doi:10.1016/B978-0-12-417197-8.00013-4
Wang D, Armstrong DL (2000) Tetraethylammonium potentiates the activity of muscarinic potassium channels in Guinea-pig atrial myocytes. J Physiol 529:699–705. doi:10.1111/j.1469-7793.2000.00699.x
Wang H, Shi H, Wang Z (1999) Nicotine depresses the functions of multiple cardiac potassium channels. Life Sci 65:PL143–PL149. doi:10.1016/S0024–3205(99)00370–7
Wang H, Shi H, Zhang L, Pourrier M, Yang B, Nattel S, Wang Z (2000a) Nicotine is a potent blocker of the cardiac A-type K(+) channels. Effects on cloned Kv4.3 channels and native transient outward current. Circulation 102:1165–1171. doi:10.1161/01.CIR.102.10.1165
Wang H, Yang B, Zhang L, Xu D, Wang Z (2000b) Direct block of inward rectifier potassium channels by nicotine. Toxicol Appl Pharmacol 164:97–101. doi:10.1006/taap.2000.8896
Zhu W, Yuan P, Shen Y, Wan R, Hong K (2016) Association of smoking with the risk of incident atrial fibrillation: a meta-analysis of prospective studies. Int J Cardiol 218:259–266. doi:10.1016/j.ijcard.2016.05.013
Acknowledgements
The authors thank Mrs. B. Vyoralová for the technical assistance.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The experiments were carried out with respect to recommendations of the European Community Guide for the Care and Use of Laboratory Animals. The experimental protocol was approved by the Local Committee for Animal Treatment at Masaryk University, Faculty of Medicine (MSMT-2526/2015-3, MSMT-4408/2016-5).
Funding
This work was supported by the Specific University Research Grant number MUNI/A/1365/2015, as provided by the Ministry of Education, Youth and Sports of the Czech Republic.
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Bébarová, M., Matejovič, P., Švecová, O. et al. Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine. Naunyn-Schmiedeberg's Arch Pharmacol 390, 471–481 (2017). https://doi.org/10.1007/s00210-017-1341-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00210-017-1341-z