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Nerolidol-loaded nanospheres prevent behavioral impairment via ameliorating Na+, K+-ATPase and AChE activities as well as reducing oxidative stress in the brain of Trypanosoma evansi-infected mice

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Abstract

The aim of this study was to investigate the effect of nerolidol-loaded nanospheres (N-NS) on the treatment of memory impairment caused by Trypanosoma evansi in mice, as well as oxidative stress, and Na+, K+-ATPase and acetylcholinesterase (AChE) activities in brain tissue. Animals were submitted to behavioral tasks (inhibitory avoidance task and open-field test) 4 days postinfection (PI). Reactive oxygen species (ROS) and thiobarbituric acid-reactive substance (TBARS) levels and catalase (CAT), superoxide dismutase (SOD), Na+, K+-ATPase and AChE activities were measured on the fifth-day PI. T. evansi-infected mice showed memory deficit, increased ROS and TBARS levels and SOD and AChE activities, and decreased CAT and Na+, K+-ATPase activities compared to uninfected mice. N-NS prevented memory impairment and oxidative stress parameters (except SOD activity), while free nerolidol (N-F) restored only CAT activity. Also, N-NS treatment was able to prevent alterations in Na+, K+-ATPase and AChE activities caused by T. evansi infection. A significantly negative correlation was observed between memory and ROS production (p < 0.001; r = −0.941), as well as between memory and AChE activity (p < 0.05; r = −0.774). On the contrary, a significantly positive correlation between memory and Na+, K+-ATPase activity was observed (p < 0.01; r = 0.844). In conclusion, N-NS was able to reverse memory impairment and to prevent increased ROS and TBARS levels due to amelioration of Na+, K+-ATPase and AChE activities and to activation of the antioxidant enzymes, respectively. These results suggest that N-NS treatment may be a useful strategy to treat memory dysfunction and oxidative stress caused by T. evansi infection.

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Matheus D. Baldissera, Carine F. Souza, Thirssa H. Grando, Andressa S. Schafer, Luciana F. Cossetin & Silvia G. Monteiro

  2. Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Karen L. S. Moreira, Marcelo L. da Veiga & Maria Izabel U. M. da Rocha

  3. Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil

    Ana P.T. da Silva

  4. Graduate School of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil

    Lenita M. Stefani & Aleksandro S. da Silva

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  1. Matheus D. Baldissera
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  2. Carine F. Souza
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  3. Thirssa H. Grando
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  9. Maria Izabel U. M. da Rocha
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Correspondence to Matheus D. Baldissera or Silvia G. Monteiro.

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Baldissera, M.D., Souza, C.F., Grando, T.H. et al. Nerolidol-loaded nanospheres prevent behavioral impairment via ameliorating Na+, K+-ATPase and AChE activities as well as reducing oxidative stress in the brain of Trypanosoma evansi-infected mice. Naunyn-Schmiedeberg's Arch Pharmacol 390, 139–148 (2017). https://doi.org/10.1007/s00210-016-1313-8

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