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The class III anti-arrhythmic agent, amiodarone, inhibits voltage-dependent K+ channels in rabbit coronary arterial smooth muscle cells

  • Hongliang Li
  • Han Sol Kim
  • Hye Won Kim
  • Sung Eun Shin
  • Won-Kyo Jung
  • Kwon-Soo Ha
  • Eun-Taek Han
  • Seok-Ho Hong
  • Amy L. Firth
  • Young Min Bae
  • Il-Whan Choi
  • Won Sun Park
Original Article

Abstract

We examined the inhibitory effect of amiodarone, a class III anti-arrhythmic agent, on voltage-dependent K+ (Kv) currents in freshly isolated rabbit coronary arterial smooth muscle cells, using a whole-cell patch clamp technique. Amiodarone inhibited Kv currents in a concentration-dependent manner, with a half-maximal inhibitory concentration (IC50) value of 3.9 ± 1.44 μM and a Hill coefficient of 0.45 ± 0.14. Amiodarone did not have a significant effect on the steady-state activation of Kv channels, but shifted the inactivation current toward a more negative potential. Application of consecutive pulses progressively augmented the amiodarone-induced Kv channel inhibition. Another class III anti-arrhythmic agent, dofetilide, did not inhibit the Kv current or change the inhibitory effect of amiodarone on Kv channels. Therefore, these results strongly suggest that amiodarone inhibits Kv currents in a concentration- and state-dependent manner.

Keywords

Amiodarone Voltage-dependent K+ channel Coronary artery 

Notes

Acknowledgments

This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Korean government (Ministry of Education: 2014-R1A1A4A01003840) (Ministry of Science, ICT and Future Planning: 2012-M3A9C7050184, 2015-R1A4A1038666).

Compliance with ethical standards

Ethical approval

All experimental procedures performed in studies involving animals were approved by the Committee for Animal Experiments of Kangwon National University.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Hongliang Li
    • 1
  • Han Sol Kim
    • 1
  • Hye Won Kim
    • 1
  • Sung Eun Shin
    • 1
  • Won-Kyo Jung
    • 2
  • Kwon-Soo Ha
    • 3
  • Eun-Taek Han
    • 4
  • Seok-Ho Hong
    • 5
  • Amy L. Firth
    • 6
  • Young Min Bae
    • 7
  • Il-Whan Choi
    • 8
  • Won Sun Park
    • 1
  1. 1.Institute of Medical Sciences, Department of PhysiologyKangwon National University School of MedicineChuncheonSouth Korea
  2. 2.Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus)Pukyong National UniversityBusanSouth Korea
  3. 3.Department of Molecular and Cellular BiochemistryKangwon National University School of MedicineChuncheonSouth Korea
  4. 4.Department of Medical Environmental Biology and Tropical MedicineKangwon National University School of MedicineChuncheonSouth Korea
  5. 5.Institute of Medical Sciences, Department of Internal MedicineKangwon National University School of MedicineChuncheonSouth Korea
  6. 6.Department of Pulmonary, Critical Care and Sleep MedicineUniversity of Southern California, Keck School of MedicineLos AngelesUSA
  7. 7.Department of PhysiologyKonkuk University School of MedicineChungjuSouth Korea
  8. 8.Department of MicrobiologyInje University College of MedicineBusanSouth Korea

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