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Effects of S-allyl cysteine on lung and liver tissue in a rat model of lipopolysaccharide-induced sepsis

Naunyn-Schmiedeberg's Archives of Pharmacology volume 388, pages 327–335 (2015)Cite this article

Abstract

Sepsis is characterized by a severe production of reactive oxygen species (ROS) and other radical species with consequent oxidative stress. S-allyl cysteine (SAC) is a water-soluble organosulfur component present in garlic which is a potent antioxidant and free radical scavenger. In the present study, the purpose was to explore the anti-inflammatory, antioxidant, and anti-apoptotic actions of SAC on lipopolysaccharide (LPS)-induced sepsis in rats. Thirty-two male Wistar rats were separated into 4 groups. These were control, SAC control, sepsis, and sepsis + SAC-induced groups. Sepsis was induced by administration of LPS (5 mg/kg) into 2 groups. SAC (50 mg/kg) was given orally to SAC control and SAC treatment groups per 12 h during 2 days after intraperitoneal LPS injection. Serum AST, ALT, ALP, and hsCRP levels and liver and lung MPO, NO, and DNA fragmentation levels were evaluated. In sepsis group, elevated levels of ALT, AST, ALP, and hsCRP were observed. The abnormal increases were decreased in sepsis + SAC group compared to sepsis group. In lung tissue, MPO and NO levels were increased in sepsis group compared to the control group. MPO activity and NO levels were decreased by SAC application in sepsis + SAC group compared with sepsis group. In liver tissue, DNA fragmentation was significantly higher in sepsis group than that in the control group. In contrast, a decreased level of DNA fragmentation was noted in sepsis + SAC group when compared with the sepsis group. In conclusion, SAC ameliorates LPS-induced indicators of liver damage and suppresses the discharge of NO and MPO in lung tissue via its antioxidant properties.

Anti-inflammatory, antioxidant and antiapoptotic effects of S-allyl cysteine in rat model of lipopolysaccharide

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Abbreviations

LPS:

Lipopolysaccharide

ROS:

Reactive oxygen species

SAC:

S-allyl cysteine

NOP:

Nitric oxide

iNOS:

Inducible NO-synthase

MPO:

Myeloperoxidase

PMNs:

Polymorphonuclear neutrophils

H&E:

Hematoxylin and eosin

SPSS:

Statistical Package for the Social Sciences

ANOVA:

One-way analysis of variance

ILs:

Interleukins

CRP:

C-reactive protein

LDL:

Low-density lipoprotein

HOCl:

Hypochlorous acid

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Acknowledgments

The authors declare no conflicts of interest in this study.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey

    Orhan Bayraktar, Ozlem Aydın & Fahrettin Akyuz

  2. Department of Chemistry, Biochemistry Division, Faculty of Science and Letters, Aksaray University, Aksaray, Turkey

    Neslihan Tekin

  3. Department of Biostatistics, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey

    Ahmet Musmul

  4. Department of Histology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey

    Dilek Burukoglu

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  1. Orhan Bayraktar
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Correspondence to Neslihan Tekin.

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Bayraktar, O., Tekin, N., Aydın, O. et al. Effects of S-allyl cysteine on lung and liver tissue in a rat model of lipopolysaccharide-induced sepsis. Naunyn-Schmiedeberg's Arch Pharmacol 388, 327–335 (2015). https://doi.org/10.1007/s00210-014-1076-z

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  • DOI: https://doi.org/10.1007/s00210-014-1076-z

Keywords

  • Lipopolysaccharide
  • S-allyl cysteine
  • Nitric oxide
  • DNA fragmentation
  • Antioxidant