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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 386, Issue 8, pp 733–745 | Cite as

Green tea extract with polyethylene glycol-3350 reduces body weight and improves glucose tolerance in db/db and high-fat diet mice

  • Jae-Hyung Park
  • Yoon Jung Choi
  • Yong Woon Kim
  • Sang Pyo Kim
  • Ho-Chan Cho
  • Shinbyoung Ahn
  • Ki-Cheor Bae
  • Seung-Soon Im
  • Jae-Hoon Bae
  • Dae-Kyu SongEmail author
Original Article

Abstract

Green tea extract (GTE) is regarded to be effective against obesity and type 2 diabetes, but definitive evidences have not been proven. Based on the assumption that the gallated catechins (GCs) in GTE attenuate intestinal glucose and lipid absorption, while enhancing insulin resistance when GCs are present in the circulation through inhibiting cellular glucose uptake in various tissues, this study attempted to block the intestinal absorption of GCs and prolong their residence time in the lumen. We then observed whether GTE containing the nonabsorbable GCs could ameliorate body weight (BW) gain and glucose intolerance in db/db and high-fat diet mice. Inhibition of the intestinal absorption of GCs was accomplished by co-administering the nontoxic polymer polyethylene glycol-3350 (PEG). C57BLKS/J db/db and high-fat diet C57BL/6 mice were treated for 4 weeks with drugs as follows: GTE, PEG, GTE + PEG, voglibose, or pioglitazone. GTE mixed with meals did not have any ameliorating effects on BW gain and glucose intolerance. However, the administration of GTE plus PEG significantly reduced BW gain, insulin resistance, and glucose intolerance, without affecting food intake and appetite. The effect was comparable to the effects of an α-glucosidase inhibitor and a peroxisome proliferator-activated receptor-γ/α agonist. These results indicate that prolonging the action of GCs of GTE in the intestinal lumen and blocking their entry into the circulation may allow GTE to be used as a prevention and treatment for both obesity and obesity-induced type 2 diabetes.

Keywords

Obesity Diabetes Green tea extract Polyethylene glycol Glucose uptake Lipid absorption 

Notes

Acknowledgments

This work was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (grant number 110135-3). Miss Sun-Joo Kim in Keimyung University School of Medicine provided valued technical assistance and care of the animals.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

210_2013_869_MOESM1_ESM.pdf (27 kb)
ESM 1 (PDF 27 kb)
210_2013_869_MOESM2_ESM.pdf (26 kb)
ESM 2 (PDF 25 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jae-Hyung Park
    • 1
  • Yoon Jung Choi
    • 3
  • Yong Woon Kim
    • 3
  • Sang Pyo Kim
    • 2
  • Ho-Chan Cho
    • 1
  • Shinbyoung Ahn
    • 4
  • Ki-Cheor Bae
    • 1
  • Seung-Soon Im
    • 1
  • Jae-Hoon Bae
    • 1
  • Dae-Kyu Song
    • 1
    Email author
  1. 1.Department of Physiology and EndocrinologyKeimyung University School of MedicineDaeguSouth Korea
  2. 2.Department of PathologyKeimyung University School of MedicineDaeguSouth Korea
  3. 3.Department of PhysiologyYeongnam University School of MedicineDaeguSouth Korea
  4. 4.GCB R&D and Clinical Trial Center Co., Ltd.SuwonSouth Korea

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