Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells

  • Hyeongseok Kim
  • Jin Young Kim
  • Hong Suk Song
  • Keon Uk Park
  • Kyo-Chul Mun
  • Eunyoung HaEmail author


This study was aimed to investigate the effect of grape seed proanthocyanidin extract (GSPE) on interleukin-17 (IL-17)-induced interleukin-6 (IL-6) production in A549 human pulmonary epithelial cells. Cells were treated with IL-17 (10 ng/ml) or GSPE (50 μg/ml), or both. The effects of GSPE on cell viability and apoptosis were evaluated. The mRNA and protein levels of IL-6 were determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Expressions of inhibitory factor κB-α (IκB-α) and ERK 1/2, p38, and JNK mitogen-activated protein kinase (MAPK) were examined by Western blot analysis. GSPE significantly inhibited both GSPE treatment only and IL-17-induced mRNA expressions and protein productions of IL-6 in pulmonary epithelial cells (p < 0.01). GSPE decreased the IL-17-induced phosphorylation of IκB-α. The IL-17 stimulated ERK 1/2, p38, and JNK MAPK activities and GSPE decreased IL-17-stimulated ERK 1/2, p38, and JNK MAPK activities. GSPE also attenuated TNF-α and IL-1β-induced IL-6 productions (p < 0.05). Our results show that GSPE may inhibit IL-17-stimulated IL-6 productions in human pulmonary epithelial cells by inhibiting MAPK and nuclear factor-κB-mediated signaling pathway.


GSPE IL-17 Pulmonary epithelial cells Inflammation IL-6 



The present research has been conducted by attached research institute research grant of Keimyung University in 2007.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Hyeongseok Kim
    • 1
  • Jin Young Kim
    • 2
  • Hong Suk Song
    • 2
  • Keon Uk Park
    • 2
  • Kyo-Chul Mun
    • 1
  • Eunyoung Ha
    • 1
    Email author
  1. 1.Department of Biochemistry, Pain Research Center, School of MedicineKeimyung UniversityDaeguRepublic of Korea
  2. 2.Department of Internal Medicine and Pain Research Center, School of MedicineKeimyung University Dongsan Medical CenterDaeguRepublic of Korea

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