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Nucleoside diphosphate kinase B is required for the formation of heterotrimeric G protein containing caveolae

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Abstract

Caveolae are flask-shaped invaginations in the plasma membrane that serve to compartmentalize and organize signal transduction processes, including signals mediated by G protein-coupled receptors and heterotrimeric G proteins. Herein we report evidence for a close association of the nucleoside diphosphate kinase B (NDPK B) and caveolin proteins which is required for G protein scaffolding and caveolae formation. A concomitant loss of the proteins NDPK B, caveolin isoforms 1 (Cav1) and 3, and heterotrimeric G proteins occurred when one of these proteins was specifically depleted in zebrafish embryos. Co-immunoprecipitation of Cav1 with the G protein Gβ-subunit and NDPK B from zebrafish lysates corroborated the direct association of these proteins. Similarly, in embryonic fibroblasts from the respective knockout (KO) mice, the membrane content of the Cav1, Gβ, and NDPK B was found to be mutually dependent on one another. A redistribution of Cav1 and Gβ from the caveolae containing fractions of lower density to other membrane compartments with higher density could be detected by means of density gradient fractionation of membranes derived from NDPK A/B KO mouse embryonic fibroblasts (MEFs) and after shRNA-mediated NDPK B knockdown in H10 cardiomyocytes. This redistribution could be visualized by confocal microscopy analysis showing a decrease in the plasma membrane bound Cav1 in NDPK A/B KO cells and vice versa and a decrease in the plasma membrane pool of NDPK B in Cav1 KO cells. Consequently, ultrastructural analysis revealed a reduction of surface caveolae in the NDPK A/B KO cells. To prove that the disturbed subcellular localization of Cav1 in NDPK A/B KO MEFs as well as NDPK B in Cav1 KO MEFs is a result of the loss of NDPK B and Cav1, respectively, we performed rescue experiments. The adenoviral re-expression of NDPK B in NDPK A/B KO MEFs rescued the protein content and the plasma membrane localization of Cav1. The expression of an EGFP-Cav1 fusion protein in Cav1-KO cells induced a restoration of NDPK B expression levels and its appearance at the plasma membrane. We conclude from these findings that NDPK B, heterotrimeric G proteins, and caveolins are mutually dependent on each other for stabile localization and caveolae formation at the plasma membrane. The data point to a disturbed transport of caveolin/G protein/NDPK B complexes from intracellular membrane compartments if one of the components is missing.

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Acknowledgments

We are grateful to H. Hosser (Anatomisches Institut, University of Heidelberg) for the expert electron microscopy analysis and E. Postel for providing the specific antibody against NDPK B. The confocal images have been captured at the Nikon Imaging Center at the University of Heidelberg. This work was supported by grants of the Deutsche Forschungsgemeinschaft to T.W. and H.J.H. (Wi1373/9-3) and the Bundesministerium für Bildung und Forschung (NGFNplus) to W.R.

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  1. Wolfgang Rottbauer

    Present address: Department of Internal Medicine II, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany

Authors and Affiliations

  1. Department of Internal Medicine III—Cardiology, University of Heidelberg, INF 410, 69120, Heidelberg, Germany

    Hans-Jörg Hippe, Steffen Just, Wolfgang Rottbauer & Hugo A. Katus

  2. Institute of Experimental and Clinical Pharmacology and Toxicology, University of Heidelberg, Maybachstrasse 14, 68169, Mannheim, Germany

    Nadine M. Wolf, H. Issam Abu-Taha & Thomas Wieland

  3. Division of Experimental Cardiology, Mannheim Medical Faculty, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany

    H. Issam Abu-Taha

  4. Department of Pharmacology, University of Göttingen, Robert-Koch-Strasse 40, 37075, Göttingen, Germany

    Susanne Lutz

  5. Centre de Recherche des Cordeliers, INSERM, U872, 75006, Paris, France

    Soazig Le Lay

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Hans-Jörg Hippe and Nadine M. Wolf contributed equally.

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Fig. S1

Specificity of used antibodies. Lysates (12 μg of protein) of MEFs (A) or zebrafish embryos 72 hpf (B) were resolved by SDS-PAGE (15% acrylamide in resolving gel) and electrically transferred onto nitrocellulose. Immunostaining was performed with anti-NDPK B (A, MC-412, Kamiya, 1:1,000; B, L-16, Santa Cruz, 1:200), anti-Cav1 (Transduction Labs, 1:1,000), anti-Cav3 (Transduction Labs, 1:2,000), anti-Gβcommon (T-20, Santa Cruz, 1:500), and anti-Gβ1 (Santa Cruz, 1:200) antibodies. Specific bands and their molecular mass are indicated (GIF 116 kb)

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Hippe, HJ., Wolf, N.M., Abu-Taha, H.I. et al. Nucleoside diphosphate kinase B is required for the formation of heterotrimeric G protein containing caveolae. Naunyn-Schmiedeberg's Arch Pharmacol 384, 461–472 (2011). https://doi.org/10.1007/s00210-011-0618-x

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