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Antiulcerogenic activity of extract, fractions, and some compounds obtained from Polygala cyparissias St. Hillaire & Moquin (Polygalaceae)

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Abstract

The present study evaluates the gastroprotective properties of acetone extract, chloroform, and methanol fractions, α-spinasterol (1); 1,3-dihydroxy-7-methoxyxanthone (2); and 1,7-dihydroxy-2,3-methylenedioxyxanthone (3) obtained from Polygala cyparissias (Polygalaceae). Gastroprotective assays were performed in mice using ethanol/HCl and nonsteroidal anti-inflammatory drug (NSAID)/bethanechol-induced ulcer models. Chloroformic fraction showed no interesting results. On the other hand, in the ethanol/HCl-induced ulcer model, the treatment using doses of 50, 125, and 250 mg/kg promoted ulcer inhibition of 45.19 ± 12.93%, 62.99 ± 3.49%, and 67.40 ± 4.75% for acetone extract and 43.70 ± 5.12%, 64.56 ± 5.64%, and 74.49 ± 6.13% for methanol fraction. In the model of NSAID/bethanechol-induced ulcer, the ulcer inhibitions in the same doses were 28.12 ± 12.45%, 60.16 ± 6.58%, and 77.86 ± 7.18% for the acetone extract and 46.09 ± 6.92%, 67.45 ± 4.36%, and 75.00 ± 2.92% for the methanol fraction. In view of the antiulcer potential of the acetone extract and its high yield and xanthone content, it was submitted to chromatographic procedures, giving compounds 1–3, which were also evaluated in the ethanol-induced ulcer model. The results showed that at a dose of 50 mg/kg, these compounds reduced the percentage of ulcer by around 71.26 ± 9.40%, 81.10 ± 5.75%, and 86.22 ± 3.42%, for compounds 1, 2, and 3, respectively. The antiulcerogenic activity of P. cyparissias may be attributed, at least in part, to these compounds.

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Acknowledgements

We are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Pesquisa Científica e Tecnológica do Estado de Santa Catarina (FAPESC), and Universidade do Vale do Itajaí (UNIVALI) for their financial support.

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Authors and Affiliations

  1. Programa de Mestrado em Ciências Farmacêuticas, Universidade do Vale do Itajaí, UNIVALI, Rua Uruguai, 458, Itajaí, Santa Catarina, 88302-202, Brazil

    Luiz Carlos Klein Jr., José Roberto Santin, Marivane Lemos, Valdir Cechinel Filho & Sérgio Faloni de Andrade

  2. Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, UNIVALI, Rua Uruguai, 458, Itajaí, Santa Catarina, 88302-202, Brazil

    Renan Becker Gandolfi, Valdir Cechinel Filho & Sérgio Faloni de Andrade

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  1. Luiz Carlos Klein Jr.
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  2. Renan Becker Gandolfi
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  3. José Roberto Santin
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  4. Marivane Lemos
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  5. Valdir Cechinel Filho
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  6. Sérgio Faloni de Andrade
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Correspondence to Sérgio Faloni de Andrade.

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Klein, L.C., Gandolfi, R.B., Santin, J.R. et al. Antiulcerogenic activity of extract, fractions, and some compounds obtained from Polygala cyparissias St. Hillaire & Moquin (Polygalaceae). Naunyn-Schmied Arch Pharmacol 381, 121–126 (2010). https://doi.org/10.1007/s00210-009-0485-x

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