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Peripheral nerve injury up-regulates expression of interactor protein for cytohesin exchange factor 1 (IPCEF1) mRNA in rat dorsal root ganglion

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Abstract

Interaction protein for cytohesin exchange factors 1 (IPCEF1) is a recently identified protein that binds to cytohesin 2 and might participate in membrane receptor trafficking by enhancing the activity of cytohesin 2 in cultured cells. However, whether IPCEF1 is involved in this process in vivo is unknown. Here, we report that IPCEF1 messenger RNA (mRNA) is expressed in the dorsal root ganglions (DRGs) of adult rats. Moreover, peripheral nerve injury induced by fifth spinal nerve ligation and transection or sciatic nerve transection significantly up-regulated expression of IPCEF1 mRNA in the injured DRGs. Because peripheral nerve injury leads to changes in membrane receptor trafficking in the injured DRG, the correlation of IPCEF1 mRNA expression with nerve injury input in the injured DRG suggests that IPCEF1 might participate in the mechanisms that underlie nerve injury-induced membrane receptor trafficking in the DRG under neuropathic pain conditions.

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Acknowledgements

This work was supported by National Institutes of Health Grants NS 058886 and NS 057343, and Johns Hopkins University Blaustein Pain Research Fund (Y.-X. T). The authors thank Claire Levine, MS, for her editorial assistance.

Authors’ contributions

XG participated in the design and interpretation of the study; carried out behavioral testing, RT-PCR, and real-time RT-PCR experiments; and wrote a draft of the manuscript. XZ was involved in the design of the study and contributed to RT-PCR and real-time RT-PCR experiments. YXT contributed to the surgery, the concept and design of the study, the data analysis and interpretation, and critical review of the manuscript.

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Authors and Affiliations

  1. Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, 367 Ross, 720 Rutland Ave., Baltimore, MD, 21205, USA

    Xiaowei Guan, Xuguang Zhu & Yuan-Xiang Tao

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  1. Xiaowei Guan
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  2. Xuguang Zhu
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  3. Yuan-Xiang Tao
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Correspondence to Yuan-Xiang Tao.

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Guan, X., Zhu, X. & Tao, YX. Peripheral nerve injury up-regulates expression of interactor protein for cytohesin exchange factor 1 (IPCEF1) mRNA in rat dorsal root ganglion. Naunyn-Schmied Arch Pharmacol 380, 459–463 (2009). https://doi.org/10.1007/s00210-009-0451-7

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  • DOI: https://doi.org/10.1007/s00210-009-0451-7

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