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The effects of reactive oxygen species on calcium- and carbachol- induced contractile responses in β-escin permeabilized rat bladder

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Abstract

The effect of reactive oxygen species on contractions in β-escin permeabilized rat detrusor was investigated. Cumulative calcium contractions were inhibited by hydrogen peroxide (H2O2) and hydroxyl (•OH) but not by superoxide (O2¯•). The sarcoplasmic reticulum calcium-ATPase inhibitor cyclopiazonic acid (CPA) and the mitochondrial blocker carbonyl cyanide p-trifluromethoxyphenylhydrazone (FCCP) decreased the calcium contractions, however in their presence, H2O2 and •OH did not have further effect. Carbachol contractions were inhibited by either H2O2/•OH/O2¯• or CPA/FCCP. In the presence of CPA, carbachol contractions were not affected by H2O2 and •OH but further decreased by O2¯•. On the other hand, only H2O2 and •OH elicited additional inhibition in carbachol responses in the presence of FCCP. Inositol triphosphate contraction was inhibited by •OH whereas none of the radicals affect carbachol induced calcium sensitization. These results show that H2O2 and •OH affects sarcoplasmic reticulum where O2¯• acts on mitochondria to change contractions in rat detrusor smooth muscle.

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Acknowledgments

This study is supported by a project grant from NOVARTIS (Turkey).

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmacology, Hacettepe University, 06100, Sıhhiye, Ankara, Turkey

    N. Tugba Durlu-Kandilci & Inci Sahin-Erdemli

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  1. N. Tugba Durlu-Kandilci
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  2. Inci Sahin-Erdemli
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Correspondence to Inci Sahin-Erdemli.

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Durlu-Kandilci, N.T., Sahin-Erdemli, I. The effects of reactive oxygen species on calcium- and carbachol- induced contractile responses in β-escin permeabilized rat bladder. Naunyn-Schmied Arch Pharmacol 378, 645–653 (2008). https://doi.org/10.1007/s00210-008-0326-3

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  • DOI: https://doi.org/10.1007/s00210-008-0326-3

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