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Effect of chronic treatment with perazine on lipopolysaccharide-induced interleukin-1β levels in the rat brain

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Abstract

In the present study, we sought to determine whether chronic treatment with perazine alters lipopolysaccharide (LPS)-induced interleukin-1β (IL-1β) levels in the following rat brain regions: the hypothalamus, frontal cortex, striatum and hippocampus. Male Wistar rats were administered perazine dimaleate (15 or 30 mg/kg/day) in drinking water for 21 days. On day 22, LPS was injected i.p. (125 μg/kg) 2 h before decapitation. Concentrations of perazine and its metabolites in plasma and brain was assessed by HPLC. The levels of IL-1β were determined using ELISA. Treatment with perazine (30 mg/kg/day) reduced LPS-stimulated IL-1β levels in the hypothalamus, and a tendency to its decrease in the striatum and frontal cortex was observed. This in vivo study suggests for the first time that long-term oral administration of perazine modulates reactivity of cells producing IL-1β.

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Acknowledgements

We wish to thank Labor (Wrocław, Poland) for a generous gift of perazine. This study was financially supported by statutory grants from the Silesian University School of Medicine and the Institute of Pharmacology, Polish Academy of Science.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Silesian University School of Medicine, Medykow 18 Street, 40-752, Katowice, Poland

    Ewa Obuchowicz, Agnieszka Marcinowska, Łukasz Drzyzga & Zbigniew S. Herman

  2. Department of Pharmacokinetics and Drug Metabolism, Polish Academy of Sciences, Institute of Pharmacology, 31-343, Kraków, Poland

    Jacek Wójcikowski & Władysława A. Daniel

Authors
  1. Ewa Obuchowicz
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  2. Agnieszka Marcinowska
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  3. Łukasz Drzyzga
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  4. Jacek Wójcikowski
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  5. Władysława A. Daniel
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  6. Zbigniew S. Herman
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Correspondence to Ewa Obuchowicz.

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Obuchowicz, E., Marcinowska, A., Drzyzga, Ł. et al. Effect of chronic treatment with perazine on lipopolysaccharide-induced interleukin-1β levels in the rat brain. Naunyn Schmied Arch Pharmacol 373, 79–84 (2006). https://doi.org/10.1007/s00210-006-0058-1

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  • DOI: https://doi.org/10.1007/s00210-006-0058-1

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