Skip to main content
SpringerLink
Log in

Potentiation of sympathetic neuromuscular transmission mediated by muscarinic receptors in guinea pig isolated vas deferens

  • Original Article
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Abstract

In guinea-pig isolated vasa deferentia, purinergic neurogenic contractions and responses to applied adenosine 5′-triphosphate (ATP) were potentiated by carbachol; responses to adrenergic transmission and applied noradrenaline were not. Following blockade of P2 receptors and α-adrenoceptors, the residual neurogenic response was massively potentiated by carbachol, suggesting the presence of a non-purinergic, non-adrenergic component. In the presence of guanethidine, carbachol had no significant effect, indicating that sympathetic transmission was the only element involved. Use of oxotremorine and selective muscarinic receptor antagonists suggested that the potentiating effect of carbachol and oxotremorine was mediated via M3 muscarinic receptors without involvement of nicotinic receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Alberts P (1995) Classification of the presynaptic muscarinic receptor subtype that regulates 3H-acetylcholine secretion in the guinea pig urinary bladder in vitro. J Pharmacol Exp Ther 274:458–468

    Google Scholar 

  • Alexander SPH, Mathie A, Peters JA (2004) Guide to receptors and channels. Br J Pharmacol 141:S6

    Google Scholar 

  • Barlow RB, Shepherd MK (1986) A further search for selective antagonists at M2-muscarinic receptors. Br J Pharmacol 89:837–842

    Google Scholar 

  • Caulfield MP, Birdsall JM (1998) International union of pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev 50:279–290

    CAS  PubMed  Google Scholar 

  • Eglen RM, Hegde SS, Watson N (1996) Muscarinic receptor subtypes and smooth muscle function. Pharmacol Rev 48:531–565

    Google Scholar 

  • Ellis JL, Burnstock G (1989) Modulation of neurotransmission in the guinea-pig vas deferens by capsaicin: involvement of calcitonin gene-related peptide and substance P. Br J Pharmacol 98:707–713

    Google Scholar 

  • Ellis JL, Burnstock G (1990) Neuropeptide Y modulation of sympathetic co-transmission in the guinea-pig vas deferens. Br J Pharmacol 100:457–462

    Google Scholar 

  • Eltze M (1988) Muscarinic M1- and M2-receptors mediating opposite effects on neuromuscular transmission in rabbit vas deferens. Eur J Pharmacol 151:205–212

    Google Scholar 

  • Hammer R, Giachetti A (1983) Muscarinic receptor subtypes M1 and M2 biochemical and functional characterization. Life Sci 31:2991–2998

    Google Scholar 

  • Hammer R, Berrie CP, Birdsall NJM, Burgen ASV, Hulme EC (1980) Pirenzepine distinguishes between subclasses of muscarinic receptors. Nature 283:90–92

    Google Scholar 

  • Hernandez M, Simonsen U, Prieto D, Rivera L, Garcia P, Ordaz E, Garcia-Sacristan A (1993) Different muscarinic receptor subtypes mediating the phasic activity and basal tone of pig isolated intravesical ureter. Br J Pharmacol 110:1413–1420

    Google Scholar 

  • Kasakov L, Burnstock G (1982) The use of the slowly degradable analog, alpha, beta-methylene ATP, to produce desensitisation of the P2-purinoceptor: effect on non-adrenergic, non-cholinergic responses of the guinea-pig urinary bladder. Eur J Pharmacol 86:291–294

    Google Scholar 

  • Kasakov L, Ellis J, Kirkpatrick K, Milner P, Burnstock G (1988) Direct evidence for concomitant release of noradrenaline, adenosine 5′-triphosphate and neuropeptide Y from sympathetic nerve supplying the guinea-pig vas deferens. J Auton Nerv Syst 22:75–82

    Google Scholar 

  • Lambrecht G, Friebe T, Grimm U, Windscheif U, Bungardt E, Hildebrandt C, Baumert HG, Spatz-Kumbel G, Mutschler E (1992) PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses. Eur J Pharmacol 217:217–219

    Google Scholar 

  • Lazareno S, Buckley NJ, Roberts FF (1990) Characterisation of muscarinic M4 binding sites in rabbit lung, chicken heart and NG 108-15 cells. Mol Pharmacol 38:805–815

    Google Scholar 

  • Lee CM (1985) Potentiation by cholinoceptor agonists of contractions to field stimulation of rat vas deferens. Br J Pharmacol 86:671–676

    Google Scholar 

  • Lundberg JM, Stjärne L (1984) Neuropeptide Y (NPY) depresses the secretion of 3H-noradrenaline and the contractile response evoked by field stimulation, in the rat vas deferens. Acta Physiol Scand 120:477–479

    Google Scholar 

  • Matsuno K, Mita S (1992) Involvement of the muscarinic receptors in the postsynaptic potentiation of neurogenic twitch contractions in the mouse vas deferens. Life Sci 50:799–806

    Google Scholar 

  • McLaren GJ, Lambrecht G, Mutschler E, Baumert HG, Sneddon P, Kennedy C (1994) Investigation of the actions of PPADS, a novel P2X-purinoceptor antagonist, in the guinea-pig isolated vas deferens. Br J Pharmacol 111:913–917

    Google Scholar 

  • Meldrum LA, Burnstock G (1983) Evidence that ATP acts as a co-transmitter with noradrenaline in sympathetic nerves supplying the guinea-pig vas deferens. Eur J Pharmacol 92:161–163

    Google Scholar 

  • Michel AD, Stefanich E, Whiting RL (1989) Direct labelling of rat M3-muscarinic receptors by [3H]4-DAMP. Eur J Pharmacol 166:459–466

    Google Scholar 

  • Sjostrand NO (1973) Effects of acetylcholine and some other smooth muscle stimulants on the electrical and mechanical responses of the guinea pig vas deferens to nerve stimulation. Acta Physiol Scand 89:1–9

    Google Scholar 

  • Sneddon P, Westfall DP (1984) Pharmacological evidence that adenosine triphosphate and noradrenaline are co-transmitters in the guinea pig vas deferens. J Physiol 347:561–580

    Google Scholar 

  • Sneddon P, McLaren GJ, Kennedy C (1996) Purinergic cotransmission: sympathetic nerves. Semin Neurosci 8:201–205

    Google Scholar 

  • Song ZM, Brookes SJH, Gibbins IL, Costa M (1994) NADPH-diaphorase and other neuronal markers in nerves and ganglia supplying the guinea pig vas deferens. J Auton Nerv Syst 48:31–43

    Google Scholar 

  • Stjernquist M, Owman C, Sjöberg NO, Sundler F (1987) Coexistence and cooperation between neuropeptide Y and norepinephrine in nerve fibres of guinea pig vas deferens and seminal vesicle. Biol Reprod 36:149–155

    Google Scholar 

  • Von Kügelgen I, Starke K (1991) Release of noradrenaline and ATP by electrical field stimulation and nicotine in guinea pig vas deferens. Naunyn-Schmiedebergs Arch Pharmacol 344:419–429

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK

    Samera Iram & Charles H. V. Hoyle

  2. Institute of Medicine, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, 1410, Brunei Darussalam

    Charles H. V. Hoyle

Authors
  1. Samera Iram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charles H. V. Hoyle
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Charles H. V. Hoyle.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iram, S., Hoyle, C.H.V. Potentiation of sympathetic neuromuscular transmission mediated by muscarinic receptors in guinea pig isolated vas deferens. Naunyn-Schmiedeberg's Arch Pharmacol 371, 212–220 (2005). https://doi.org/10.1007/s00210-004-1015-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00210-004-1015-5

Keywords

Search

Navigation