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Heat-shock response is associated with decreased production of interleukin-6 in murine aortic vascular smooth muscle cells

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Abstract

Heat shock has been known to change cellular responses to noxious stimuli by inducing heat-shock proteins (Hsps). We hypothesized that a heat-shock response modulates cytokine production in murine aortic vascular smooth muscle cells (VSMCs). VSMCs were exposed to 44°C for 15–60 min, and subjected to interleukin-1β (IL-1β) or tumor necrosis factor α (TNFα), which induced interleukin-6 (IL-6) production. Expression of Hsps was examined with immunoblots, immunocytochemistry, or enzyme-linked immunosorbent assay (ELISA), and that of IL-6 with reverse transcription-polymerase chain reaction (RT-PCR) or ELISA. Heat shock (44°C for 45 min) induced Hsp72 in VSMCs at 4 h and elicited its maximal expression at 8 h after the end of heat shock. Treatment with IL-1β increased IL-6 transcription in VSMCs up to 24 h in an incubation time-dependent manner. Treatment with IL-1β or TNFα caused a concentration-dependent increase in IL-6 production in culture medium, which was attenuated by heat shock. Although treatment with Hsp72 or Hsp60 alone did not significantly affect basal IL-6 release into culture medium statistically, cotreatment with IL-1β and Hsp72, but not Hsp60 or boiled Hsp72, decreased IL-1β-induced IL-6 production in culture medium. Introduction of Hsp72, but not Hsp60, into VSMCs decreased IL-1β-induced IL-6 production in culture medium. These results indicate that the heat-shock response transcriptionally attenuated production of IL-6 in murine aortic VSMCs.

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Authors and Affiliations

  1. Department of Pharmacology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    InKyeom Kim

  2. Department of Physiology, Dongguk University College of Oriental Medicine, Kyongju, Kyongbuk, 780-714, Republic of Korea

    Heung-Mook Shin

  3. Department of Anesthesiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Woonyi Baek

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  1. InKyeom Kim
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Correspondence to InKyeom Kim.

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Kim, I., Shin, HM. & Baek, W. Heat-shock response is associated with decreased production of interleukin-6 in murine aortic vascular smooth muscle cells. Naunyn-Schmiedeberg's Arch Pharmacol 371, 27–33 (2005). https://doi.org/10.1007/s00210-004-1007-5

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