Diazepam potentiates the effects of endogenous catecholamines on contractility and cyclic AMP levels in rat ventricular myocardium

Abstract.

Diazepam has phosphodiesterase (PDE) inhibitory activity and potentiates the effect of some 3',5'-cyclic adenosine monophosphate (cAMP)-dependent positive inotropic agents. The present study was undertaken to determine whether diazepam enhances the contractile responses and cAMP levels induced by endogenous catecholamines in electrically driven rat right ventricular strips, and the effects are compared with that of the PDE inhibitor 3-isobutylmethylxantine (IBMX). Noradrenaline (10 nM–1 µM), adrenaline (50 nM–500 µM) and tyramine (5–100 µM) produced concentration-dependent positive inotropic effects that were potentiated by the presence of 10 µM diazepam or IBMX. The diazepam-induced potentiation of the contractile effect of the sympathomimetic agents was not mimicked by 100 µM GABA nor was it antagonized by a 5 µM concentration of the blockers of central and peripheral type benzodiazepine receptors, flumazenil and PK 11195. The β₂-adrenergic receptor agonist salbutamol (0.1–300 µM) also produced a concentration-dependent positive inotropic effect which was potentiated by the presence of 10 µM diazepam or 10 µM IBMX. However, the contractile effect of salbutamol, either alone or in the presence of diazepam or IBMX, was not affected by 50 nM ICI 118551, an antagonist of β₂-adrenergic receptors, but was virtually abolished by a 0.3 µM concentration of CGP 20712A, an antagonist of β₁-adrenergic receptors. Diazepam and IBMX also potentiated the increase in cAMP levels caused by these three sympathomimetic agents in this tissue. [³H]Noradrenaline release elicited by electrical stimulation or by tyramine was not affected by diazepam. The results demonstrate that diazepam, like the phosphodiesterase inhibitor IBMX, produces an inotropic and biochemical potentiation of the effects of endogenous catecholamines in rat myocardium. This effect is not due to the release of noradrenaline at the presynaptic level nor is it mediated by β₂-adrenergic receptors or benzodiazepine receptors of the central or peripheral type. The effect is probably consequential upon the phosphodiesterase inhibitory activity of diazepam.