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Inflammation-associated suppression of metabolic gene networks in acute and chronic liver disease

Archives of Toxicology volume 94, pages 205–217 (2020)Cite this article

Abstract

Inflammation has been recognized as essential for restorative regeneration. Here, we analyzed the sequential processes during onset of liver injury and subsequent regeneration based on time-resolved transcriptional regulatory networks (TRNs) to understand the relationship between inflammation, mature organ function, and regeneration. Genome-wide expression and TRN analysis were performed time dependently in mouse liver after acute injury by CCl4 (2 h, 8 h, 1, 2, 4, 6, 8, 16 days), as well as lipopolysaccharide (LPS, 24 h) and compared to publicly available data after tunicamycin exposure (mouse, 6 h), hepatocellular carcinoma (HCC, mouse), and human chronic liver disease (non-alcoholic fatty liver, HBV infection and HCC). Spatiotemporal investigation differentiated lobular zones for signaling and transcription factor expression. Acute CCl4 intoxication induced expression of gene clusters enriched for inflammation and stress signaling that peaked between 2 and 24 h, accompanied by a decrease of mature liver functions, particularly metabolic genes. Metabolism decreased not only in pericentral hepatocytes that underwent CCl4-induced necrosis, but extended to the surviving periportal hepatocytes. Proliferation and tissue restorative TRNs occurred only later reaching a maximum at 48 h. The same upstream regulators (e.g. inhibited RXR function) were implicated in increased inflammation and suppressed metabolism. The concomitant inflammation/metabolism TRN occurred similarly after acute LPS and tunicamycin challenges, in chronic mouse models and also in human liver diseases. Downregulation of metabolic genes occurs concomitantly to induce inflammation-associated genes as an early response and appears to be initiated by similar upstream regulators in acute and chronic liver diseases in humans and mice. In the acute setting, proliferation and restorative regeneration associated TRNs peak only later when metabolism is already suppressed.

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Abbreviations

HCC:

Hepatocellular carcinoma

NAFLD:

Non-alcoholic fatty acid liver disease

TRN:

Transcriptional regulatory networks

IPA:

Ingenuity pathway analysis

Tm:

Tunicamycin

LPS:

Lipopolysaccharide

HBV:

Hepatitis virus type B

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Acknowledgements

We thank Ms. Katharina Rochlitz, Ms. Brigitte Begher-Tibbe, and Ms. Georgia Günther (Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund, Germany) for excellent technical assistance. This work has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 681002 (EU-ToxRisk), from TransQST (No. 116030), LivSysTransfer (BMBF, 031L0119) with contributions from StemCellNet (BMBF, 01EK1604A), LiSyM (BMBF, 031Loo45), and InnoSysTox (BMBF/EU, 031L0021A) We thank the Permanent Senate Commission on Food Safety (SKLM) for valuable discussion.

Funding

This study was supported by the European Union Seventh Framework Programme (FP7) Health projects DETECTIVE (EU-project FP7-Health Grant agreement no. 266838) and NOTOX (EU-project FP7-Health Grant agreement no. 267038) (JGH), the DFG WISP (GO 1987/2-1) (PG), the BMBF (German Federal Ministry of Education and Research) project Virtual Liver (0313854) (JGH), FONDECYT no. 1140549, Millennium Institute no. P09-015-F and FONDAP 15150012 (CH).

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Author notes

  1. Gisela Campos and Wolfgang Schmidt-Heck equal contribution.

Authors and Affiliations

  1. IfADo-Leibniz Research Centre for Working Environment and Human Factors, Technical University Dortmund, Ardeystrasse 67, 44139, Dortmund, Germany

    Gisela Campos, Agata Widera, Ahmed Ghallab, Larissa Pütter, Daniela González, Karolina Edlund, Cristina Cadenas, Rosemarie Marchan, Jan G. Hengstler & Patricio Godoy

  2. Leibniz Institute for Natural Product Research and Infection Biology e.V., Hans-Knöll Institute, Jena, Germany

    Wolfgang Schmidt-Heck & Reinhard Guthke

  3. Stem Cell Institute, KU Leuven, Leuven, Belgium

    Jonathan De Smedt & Catherine Verfaillie

  4. Department of Forensic and Veterinary Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt

    Ahmed Ghallab

  5. Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago, Chile

    Claudio Hetz

  6. Center for Geroscience, Brain Health and Metabolism (GERO), Santiago, Chile

    Claudio Hetz

  7. The Buck Institute for Research in Aging, Novato, CA, 94945, USA

    Claudio Hetz

  8. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA

    Claudio Hetz

  9. Medical Faculty, Institute of Neurophysiology, University of Cologne, Cologne, Germany

    Agapios Sachinidis

  10. Department of Experimental and Clinical Pharmacology and Toxicology, University of Tübingen, Tübingen, Germany

    Albert Braeuning & Michael Schwarz

  11. Department of Food Safety, Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany

    Albert Braeuning

  12. Department of Pediatrics and Juvenile Medicine, Center for Liver Cell Research, University of Regensburg Hospital, Regensburg, Germany

    Thomas S. Weiß

  13. Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA

    Benjamin K. Banhart, Jan Hoek & Rajanikanth Vadigepalli

  14. Vertex Pharmaceuticals, 3215 Merryfield Row, San Diego, CA, 92121, USA

    Jeffrey Willy

  15. Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46285, USA

    James L. Stevens

  16. MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, E16 4UU, UK

    David C. Hay

Authors
  1. Gisela Campos
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  24. Jan G. Hengstler
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Contributions

GC, AW, AG, LP mouse experiments with CCl4, Tm and LPS challenge; GC, AW, LP western blot analyses; GC histological analysis; WS-H, PG, JS and AS gene array analysis and bioinformatics; AB and MS zonated gene array analysis; GC, DG real-time PCR analysis; GC, JGH and PG drafting of manuscript, acquisition of funding; GC, JGH and PG Study concept and design, critical review of manuscript, study supervision; KE, CC, RM, RR, RG, CV, CH, MS, TSW, JLS, DCH and DD study concept and design, critical revision of manuscript.

Corresponding authors

Correspondence to Jan G. Hengstler or Patricio Godoy.

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Campos, G., Schmidt-Heck, W., De Smedt, J. et al. Inflammation-associated suppression of metabolic gene networks in acute and chronic liver disease. Arch Toxicol 94, 205–217 (2020). https://doi.org/10.1007/s00204-019-02630-3

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Keywords

  • Liver injury
  • Regeneration
  • Transcriptomics
  • Gene networks