Archives of Toxicology

, Volume 89, Issue 11, pp 2079–2087

The influence of chronic l-carnitine supplementation on the formation of preneoplastic and atherosclerotic lesions in the colon and aorta of male F344 rats

  • Michael T. Empl
  • Patricia Kammeyer
  • Reiner Ulrich
  • Jan F. Joseph
  • Maria K. Parr
  • Ina Willenberg
  • Nils H. Schebb
  • Wolfgang Baumgärtner
  • Elke Röhrdanz
  • Christian Steffen
  • Pablo Steinberg
Organ Toxicity and Mechanisms

DOI: 10.1007/s00204-014-1341-4

Cite this article as:
Empl, M.T., Kammeyer, P., Ulrich, R. et al. Arch Toxicol (2015) 89: 2079. doi:10.1007/s00204-014-1341-4

Abstract

l-Carnitine, a key component of fatty acid oxidation, is nowadays being extensively used as a nutritional supplement with allegedly “fat burning” and performance-enhancing properties, although to date there are no conclusive data supporting these claims. Furthermore, there is an inverse relationship between exogenous supplementation and bioavailability, i.e., fairly high oral doses are not fully absorbed and thus a significant amount of carnitine remains in the gut. Human and rat enterobacteria can degrade unabsorbed l-carnitine to trimethylamine or trimethylamine-N-oxide, which, under certain conditions, may be transformed to the known carcinogen N-nitrosodimethylamine. Recent findings indicate that trimethylamine-N-oxide might also be involved in the development of atherosclerotic lesions. We therefore investigated whether a 1-year administration of different l-carnitine concentrations (0, 1, 2 and 5 g/l) via drinking water leads to an increased incidence of preneoplastic lesions (so-called aberrant crypt foci) in the colon of Fischer 344 rats as well as to the appearance of atherosclerotic lesions in the aorta of these animals. No significant difference between the test groups regarding the formation of lesions in the colon and aorta of the rats was observed, suggesting that, under the given experimental conditions, l-carnitine up to a concentration of 5 g/l in the drinking water does not have adverse effects on the gastrointestinal and vascular system of Fischer 344 rats.

Keywords

l-Carnitine Trimethylamine Aberrant crypt foci F344 rat Atherosclerosis 

Supplementary material

204_2014_1341_MOESM1_ESM.pdf (104 kb)
Supplementary material 1 (PDF 103 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Michael T. Empl
    • 1
  • Patricia Kammeyer
    • 2
  • Reiner Ulrich
    • 2
  • Jan F. Joseph
    • 4
  • Maria K. Parr
    • 4
  • Ina Willenberg
    • 1
  • Nils H. Schebb
    • 1
  • Wolfgang Baumgärtner
    • 2
  • Elke Röhrdanz
    • 3
  • Christian Steffen
    • 3
  • Pablo Steinberg
    • 1
  1. 1.Institute for Food Toxicology and Analytical ChemistryUniversity of Veterinary Medicine HannoverHannoverGermany
  2. 2.Department of PathologyUniversity of Veterinary Medicine HannoverHannoverGermany
  3. 3.Federal Institute for Drugs and Medical DevicesBonnGermany
  4. 4.Institute of PharmacyFree University of BerlinBerlinGermany

Personalised recommendations