Abstract
The objective of the current study was to investigate the effect of vanadium (V5+) on Cyp1 expression and activity in C57BL/6 mice liver and isolated hepatocytes. For this purpose, C57BL6 mice were injected intraperitoneally with V5+ (5 mg/kg) in the absence and presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (15 μg/kg) for 6 and 24 h. Furthermore, isolated hepatocytes from C57BL6 mice were treated with V5+ (5, 10, and 20 μM) in the absence and presence of TCDD (1 nM) for 3, 6, 12, and 24 h. In vivo, V5+ alone did not significantly alter Cyp1a1, Cyp1a2, or Cyp1b1 mRNA, protein, or catalytic activity levels. Upon co-exposure to V5+ and TCDD, V5+ significantly potentiated the TCDD-mediated induction of the Cyp1a1, Cyp1a2, and Cyp1b1 mRNA, protein, and catalytic activity levels at 24 h. In vitro, V5+ decreased the TCDD-mediated induction of Cyp1a1 mRNA, protein, and catalytic activity levels. Furthermore, V5+ significantly inhibited the TCDD-induced AhR-dependent luciferase activity. V5+ also increased serum hemoglobin (Hb) levels in animals treated for 24 h. Upon treatment of isolated hepatocytes with Hb alone or in the presence of TCDD, there was an increase in the AhR-dependent luciferase activity. When isolated hepatocytes were treated for 2 h with V5+ in the presence of TCDD, followed by replacement of the medium with new medium containing Hb, there was further potentiation to the TCDD-mediated effect. The present study demonstrates that there is a differential modulation of Cyp1a1 by V5+ in C57BL/6 mice livers and isolated hepatocytes and demonstrates Hb as an in vivo specific modulator.
Similar content being viewed by others
Abbreviations
- AhR:
-
Aryl hydrocarbon receptor
- V5+ :
-
Vanadium
- Cyp450s:
-
Cytochrome P450s
- HO-1:
-
Heme oxygenase-1
- TCDD:
-
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
- XRE:
-
Xenobiotic responsive element
References
Abdelhamid G, Anwar-Mohamed A, Badary OA, Moustafa AA, El-Kadi AO (2010a) Transcriptional and posttranscriptional regulation of CYP1A1 by vanadium in human hepatoma HepG2 cells. Cell Biol Toxicol 26(5):421–434. doi:10.1007/s10565-010-9153-7
Abdelhamid G, Anwar-Mohamed A, Elmazar MM, El-Kadi AO (2010b) Modulation of NAD(P)H:quinone oxidoreductase by vanadium in human hepatoma HepG2 cells. Toxicol In Vitro Int J Publ Assoc BIBRA 24(6):1554–1561. doi:10.1016/j.tiv.2010.06.017
Abdelhamid G, Amara IE, Anwar-Mohamed A, El-Kadi AO (2012) Modulation of aryl hydrocarbon receptor-regulated genes by acute administration of ammonium metavanadate in kidney, lung and heart of C57BL/6 mice. J App Toxicol JAT doi:10.1002/jat.2774
Amara IE, Anwar-Mohamed A, Abdelhamid G, El-Kadi AO (2012) Mercury modulates the cytochrome P450 1a1, 1a2 and 1b1 in C57BL/6 mice: in vivo and in vitro studies. Toxicol Appl Pharmacol 266(3):419–429. doi:10.1016/j.taap.2012.11.027
Amoyal I, Prus E, Fibach E (2007) Vanadate elevates fetal hemoglobin in human erythroid precursors by inhibiting cell maturation. Exp Biol Med (Maywood) 232(5):654–661
Anwar-Mohamed A, El-Kadi AO (2008) Down-regulation of the carcinogen-metabolizing enzyme cytochrome P450 1a1 by vanadium. Drug Metab Dispos Biol Fate Chem 36(9):1819–1827. doi:10.1124/dmd.108.021154
Anwar-Mohamed A, El-Kadi AO (2009) Down-regulation of the detoxifying enzyme NAD(P)H:quinone oxidoreductase 1 by vanadium in Hepa 1c1c7 cells. Toxicol Appl Pharmacol 236(3):261–269
Anwar-Mohamed A, El-Kadi AO (2010) Arsenite down-regulates cytochrome P450 1A1 at the transcriptional and posttranslational levels in human HepG2 cells. Free Radical Biol Med 48(10):1399–1409. doi:10.1016/j.freeradbiomed.2010.02.027
Barakat MM, El-Kadi AO, du Souich P (2001) L-NAME prevents in vivo the inactivation but not the down-regulation of hepatic cytochrome P450 caused by an acute inflammatory reaction. Life Sci 69(13):1559–1571
Baran EJ (2008) Vanadium detoxification: chemical and biochemical aspects. Chem Biodivers 5(8):1475–1484
Barceloux DG (1999) Vanadium. J Toxicol Clin Toxicol 37(2):265–278
Beyhl FE, Mayer DG (1983) Effects of ammonium metavanadate on rat liver enzymes. Toxicol Lett 19(1–2):81–85
Brauze D, Widerak M, Cwykiel J, Szyfter K, Baer-Dubowska W (2006) The effect of aryl hydrocarbon receptor ligands on the expression of AhR, AhRR, ARNT, Hif1alpha, CYP1A1 and NQO1 genes in rat liver. Toxicol Lett 167(3):212–220
Byrne AR, Kosta L (1978) Vanadium in foods and in human body fluids and tissues. Sci Total Environ 10(1):17–30
Dafnis E, Spohn M, Lonis B, Kurtzman NA, Sabatini S (1992) Vanadate causes hypokalemic distal renal tubular acidosis. Am J Physiol 262(3 Pt 2):F449–F453
El-Kadi AO, Maurice H, Ong H, du Souich P (1997) Down-regulation of the hepatic cytochrome P450 by an acute inflammatory reaction: implication of mediators in human and animal serum and in the liver. Br J Pharmacol 121(6):1164–1170
Evangelou AM (2002) Vanadium in cancer treatment. Crit Rev Oncol Hematol 42(3):249–265
Galloway JT, Norton J, Volchok S, MacLean H (1982) Trace metals in atmospheric deposition: a review and assessment. Atmos Environ 16:1677–1700
Germolec DR, Henry EC, Maronpot R et al (1996) Induction of CYP1A1 and ALDH-3 in lymphoid tissues from Fisher 344 rats exposed to 2,3,7,8-tetrachlorodibenzodioxin (TCDD). Toxicol Appl Pharmacol 137(1):57–66
Guengerich FP, Shimada T (1998) Activation of procarcinogens by human cytochrome P450 enzymes. Mutat Res 400(1–2):201–213
Hankinson O (1995) The aryl hydrocarbon receptor complex. Annu Rev Pharmacol Toxicol 35:307–340
Hansen TV, Aaseth J, Skaug V (1986) Hemolytic activity of vanadyl sulphate and sodium vanadate. Acta Pharmacologica et Toxicologica 59(Suppl 7):562–565
Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48(2):422–427
Hogan GR (1990) Peripheral erythrocyte levels, hemolysis and three vanadium compounds. Experientia 46(5):444–446
Klimecki WT, Carter DE (1995) Arsine toxicity: chemical and mechanistic implications. J Toxicol Environ Health 46(4):399–409. doi:10.1080/15287399509532045
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402–408
Lorenzen A, Kennedy SW (1993) A fluorescence-based protein assay for use with a microplate reader. Anal Biochem 214(1):346–348
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275
Mamane Y, Pirrone N (1998) Vanadium in the atmosphere, vol Part 1: chemistry and biochemistry. Wiley, New York, NY
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63
Nebert DW, Eisen HJ, Hankinson O (1984) The Ah receptor: binding specificity only for foreign chemicals? Biochem Pharmacol 33(6):917–924
Obianime AW, Gogo-Abite M, Roberts II (2009) The effects of ammonium metavanadate on biochemical, hormonal, haematological and histopathological parameters of the female Wistar rats. Niger J Physiol Sci Off Publ Physiol Soc Niger 24(2):187–194
Pohl RJ, Fouts JR (1980) A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Anal Biochem 107(1):150–155
Roman RJ, Bonventre JV, Silva P, Lechene C (1981) Sodium orthovanadate diuresis in rats. J Pharmacol Exp Ther 218(1):168–174
Sambrook J, Fritsch EF, Maniatatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Plainview, NY
Seglen PO (1976) Preparation of isolated rat liver cells. Methods Cell Biol 13:29–83
Setyawati IA, Thompson KH, Yuen VG et al (1998) Kinetic analysis and comparison of uptake, distribution, and excretion of 48 V-labeled compounds in rats. J Appl Physiol 84(2):569–575
Spink DC, Katz BH, Hussain MM et al (2002) Induction of CYP1A1 and CYP1B1 in T-47D human breast cancer cells by benzo[a]pyrene is diminished by arsenite. Drug Metab Dispos 30(3):262–269
Talvitie NA, Wagner WD (1954) Studies in vanadium toxicology. II. Distribution and excretion of vanadium in animals. AMA Arch Ind Hyg Occup Med 9(5):414–422
Uno S, Dragin N, Miller ML, Dalton TP, Gonzalez FJ, Nebert DW (2008) Basal and inducible CYP1 mRNA quantitation and protein localization throughout the mouse gastrointestinal tract. Free Radic Biol Med 44(4):570–583
Vakharia DD, Liu N, Pause R et al (2001) Effect of metals on polycyclic aromatic hydrocarbon induction of CYP1A1 and CYP1A2 in human hepatocyte cultures. Toxicol Appl Pharmacol 170(2):93–103. doi:10.1006/taap.2000.9087
Vazquez A, Tudela J, Varon R, Garcia-Canovas F (1991) Determination of hemoglobin through its peroxidase activity on chlorpromazine. J Biochem Biophys Methods 23(1):45–52
Volotinen M, Maenpaa J, Kankuri E et al (2009) Expression of cytochrome P450 (CYP) enzymes in human nonpigmented ciliary epithelial cells: induction of CYP1B1 expression by TCDD. Invest Ophthalmol Vis Sci 50(7):3099–3105
Yang CS, Smith TJ, Hong JY (1994) Cytochrome P-450 enzymes as targets for chemoprevention against chemical carcinogenesis and toxicity: opportunities and limitations. Cancer Res 54(7 Suppl):1982s–1986s
Acknowledgments
This work was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant RGPIN 250139-07 to A.O.S. I.E.A is the recipient of Libyan Government Scholarship. A.A-M. is the recipient of Alberta Ingenuity Graduate Scholarship and Izaak Walton Killam memorial graduate Scholarship.
Conflict of interest
The authors declare no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Abdelhamid, G., Amara, I.E.A., Anwar-Mohamed, A. et al. Modulation of cytochrome P450 1 (Cyp1) by vanadium in hepatic tissue and isolated hepatocyte of C57BL/6 mice. Arch Toxicol 87, 1531–1543 (2013). https://doi.org/10.1007/s00204-013-1023-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00204-013-1023-7