Several in vitro DNA microarray studies have shown the importance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in skin sensitization. Nevertheless, the exact in vivo role of the Nrf2-Keap1 pathway during the induction of skin sensitization remains unknown. To study the function of Nrf2, a local lymph node assay was performed in wild-type and Nrf2-deficient mice using 2,4-dinitrochlorobenzene. The Nrf2-deficient mice show a more pronounced response, indicating that Nrf2 is involved in dampening the induction of skin sensitization.
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We acknowledge Ilse Tonk for her expertise in the benchmark dose approach. Dirk Elberts and Tanja Schouten are acknowledged for their technical assistance in breeding the mice and performing the dissections. We thank Dr. S Werner and Dr. T Ramadan of the Institute of Cell biology in Zurich for their generous gift of the Nrf2-deficient mice. The Dutch Ministry of Health, Welfare and Sports and the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (NWO) (grant no: 050-060-510) are acknowledged for funding this work.
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van der Veen, J.W., Gremmer, E.R., Vermeulen, J.P. et al. Induction of skin sensitization is augmented in Nrf2-deficient mice. Arch Toxicol 87, 763–766 (2013). https://doi.org/10.1007/s00204-012-0976-2
- Skin sensitization
- In vivo