Archives of Toxicology

, Volume 81, Issue 9, pp 665–673 | Cite as

Pre- and postnatal toxicity of the commercial glyphosate formulation in Wistar rats

  • Eliane DallegraveEmail author
  • Fabiana D. Mantese
  • Rosemari T. Oliveira
  • Anderson J. M. Andrade
  • Paulo R. Dalsenter
  • Augusto Langeloh
Reproductive Toxicology


Glyphosate is the active ingredient and polyoxyethyleneamine is the surfactant present in the herbicide Roundup® formulation commercialized in Brazil. The aim of this study was to assess the reproductive effects of glyphosate-Roundup® on male and female offspring of Wistar rats exposed during pregnancy and lactation. Dams were treated orally with water or 50, 150 or 450 mg/kg glyphosate during pregnancy (21–23 days) and lactation (21 days). These doses do not correspond to human exposure levels. The results showed that glyphosate-Roundup® did not induce maternal toxicity but induced adverse reproductive effects on male offspring rats: a decrease in sperm number per epididymis tail and in daily sperm production during adulthood, an increase in the percentage of abnormal sperms and a dose-related decrease in the serum testosterone level at puberty, and signs of individual spermatid degeneration during both periods. There was only a vaginal canal-opening delay in the exposed female offspring. These findings suggest that in utero and lactational exposure to glyphosate-Roundup® may induce significant adverse effects on the reproductive system of male Wistar rats at puberty and during adulthood.


Male reproductive effects Glyphosate Polyoxyethyleneamine Roundup® Wistar rats 



We are grateful to CAPES and FAPERGS for financial support. We also thank Dr. Rosana Nogueira de Morais for assistance with the radioimmunoassays.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Eliane Dallegrave
    • 1
    Email author
  • Fabiana D. Mantese
    • 1
  • Rosemari T. Oliveira
    • 2
  • Anderson J. M. Andrade
    • 3
  • Paulo R. Dalsenter
    • 3
  • Augusto Langeloh
    • 1
  1. 1.Department of PharmacologyFederal University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Departament of PathologyFederal University of Rio Grande do SulPorto AlegreBrazil
  3. 3.Department of PharmacologyFederal University of ParanáCuritibaBrazil

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