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Pulmonary exposure to diesel exhaust particles induces airway inflammation and cytokine expression in NC/Nga mice

Archives of Toxicology volume 79, pages 595–599 (2005)Cite this article

Abstract

Although several studies have reported that diesel exhaust particles (DEP) affect cardiorespiratory health in animals and humans, the effect of DEP on animal models with spontaneous allergic disorders has been far less intensively studied. The Nc/Nga mouse is known to be a typical animal model for human atopic dermatitis (AD). In the present study, we investigated the effects of repeated pulmonary exposure to DEP on airway inflammation and cytokine expression in NC/Nga mice. The animals were randomized into two experimental groups that received vehicle or DEP by intratracheal instillation weekly for six weeks. Cellular profiles of bronchoalveolar lavage (BAL) fluid and expressions of cytokines and chemokines in both the BAL fluid and lung tissues were evaluated 24 h after the last instillation. The DEP challenge produced an increase in the numbers of total cells, neutrophils, and mononuclear cells in BAL fluid as compared to the vehicle challenge (P<0.01). DEP exposure significantly induced the lung expressions of interleukin (IL)-4, keratinocyte chemoattractant (KC), and macrophage inflammatory protein (MIP)-1α when compared to the vehicle challenge. These results indicate that intratracheal exposure to DEP induces the recruitment of inflammatory cells, at least partially, through the local expression of IL-4 and chemokines in NC/Nga mice.

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Affiliations

  1. Inhalation Toxicology and Pathophysiology Research Team, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan

    Ken-ichiro Inoue, Hirohisa Takano & Rie Yanagisawa

  2. Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Hirohisa Takano & Toshikazu Yoshikawa

  3. Department of Health Science, Oita University of Nursing and Health Science, Oita, Japan

    Takamichi Ichinose

  4. Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Tottori, Japan

    Akinori Shimada

Authors
  1. Ken-ichiro Inoue
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  2. Hirohisa Takano
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  3. Rie Yanagisawa
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  4. Takamichi Ichinose
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  5. Akinori Shimada
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  6. Toshikazu Yoshikawa
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Correspondence to Hirohisa Takano.

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Inoue, Ki., Takano, H., Yanagisawa, R. et al. Pulmonary exposure to diesel exhaust particles induces airway inflammation and cytokine expression in NC/Nga mice. Arch Toxicol 79, 595–599 (2005). https://doi.org/10.1007/s00204-005-0668-2

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  • DOI: https://doi.org/10.1007/s00204-005-0668-2

Keywords

  • NC/Nga mice
  • DEP
  • Cytokine
  • Chemokine