Abstract
Prominent low-molecular-weight proteins were isolated from vegetative cells of the myxobacterium Stigmatella aurantiaca and were found to be members of the cold-shock protein family. A first gene of this family (cspA) was cloned and sequenced. It encodes a protein of 68 amino acid residues that displays up to 71% sequence identity with other bacterial cold-shock(-like) proteins. A cysteine residue within the RNP-2 motif is a peculiarity of Stigmatella CspA. A cspA::(Δtrp-lacZ) fusion gene construct was introduced into Stigmatella by electroporation, a method that has not been used previously for this strain. Analysis of the resultant transformants revealed that cspA transcription occurs at high levels during vegetative growth at 20 and 32 °C, and during fruiting body formation.
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Received: 15 January 1999 / Accepted: 1 June 1999
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Stamm, I., Leclerque, A. & Plaga, W. Purification of cold-shock-like proteins from Stigmatella aurantiaca– molecular cloning and characterization of the cspA gene. Arch Microbiol 172, 175–181 (1999). https://doi.org/10.1007/s002030050757
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DOI: https://doi.org/10.1007/s002030050757