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Archives of Microbiology

, Volume 166, Issue 3, pp 176–183 | Cite as

Ruminococcus hydrogenotrophicus sp. nov., a new H2/CO2-utilizing acetogenic bacterium isolated from human feces

  • A. Bernalier
  • Anne Willems
  • Marion Leclerc
  • Violaine Rochet
  • Matthew D. Collins
Original paper

Abstract

A new H2/CO2-utilizing acetogenic bacterium was isolated from the feces of a non-methane-excreting human subject. The two strains S5a33 and S5a36 were strictly anaerobic, gram-positive, non-sporulating coccobacilli. The isolates grew autotrophically by metabolizing H2/CO2 to form acetate as sole metabolite and were also able to grow heterotrophically on a variety of organic compounds. The major end product of glucose and fructose fermentation was acetate; the strains also formed ethanol, lactate and, to a lesser extent, isobutyrate and isovalerate. The G+C content of DNA of strain S5a33 was 45.2 mol%. 16S rRNA gene sequencing demonstrated that the two acetogenic isolates were phylogenetically identical and represent a new subline within Clostridium cluster XIVa. Based on phenotypic and phylogenetic considerations, a new species, Ruminococcus hydrogenotrophicus, is proposed. The type strain of R. hydrogenotrophicus is S5a33 (DSM 10507). Furthermore, H2/CO2 acetogenesis appeared to be a common property of most of the species phylogenetically closely related to strain S5a33 (Clostridium coccoides, Ruminococcus hansenii, and Ruminococcus productus).

Key wordsRuminococcus hydrogenotrophicus Human feces Anaerobic bacteria Acetogenesis H2/CO2-utilizing acetogens Phylogeny 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • A. Bernalier
    • 1
  • Anne Willems
    • 2
  • Marion Leclerc
    • 1
  • Violaine Rochet
    • 1
  • Matthew D. Collins
    • 2
  1. 1.Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, F-78352 Jouy-en-Josas Cedex, France Tel. +33-134-652709; Fax +33-134-652311 e-mail: bernalie@jouy.inra.fr.FR
  2. 2.Microbiology Department, Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UKGB

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