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Identification of activities that catalyze the cis-trans isomerization of the double bond of a mono-unsaturated fatty acid in Pseudomonas sp. strain E-3

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Abstract

A cell-free extract of Pseudomonas sp. strain E-3 catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). The cytosolic fraction catalyzed the isomerizations of free 16:1(9c) by itself and of 16:1(9c) esterified to PE in the presence of the membrane fraction. Tracer experiments using [2,2-2H2]16:1(9c) demonstrated that the isomerization of free 16:1(9c) occurred independently of the isomerization of 16:1(9c) esterified to PE, indicating that this bacterium has two types of activities that catalyze the cis-trans isomerization of the double bond of a mono-unsaturated fatty acid.

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Authors and Affiliations

  1. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060, Japan Fax +81-11-757-5994 e-mail: hoku@bio.hokudai.ac.jp, , , , , , JP

    H. Okuyama & Daisuke Enari

  2. Department of Clinical Nutrition, Osaka Prefectural College of Health Sciences, Habikino 583, Japan, , , , , , JP

    Akira Shibahara

  3. General Testing Research Institute of Japan Oil Stuff Inspectors’ Corporation, Higashinada-ku, Kobe 658, Japan, , , , , , JP

    Kohei Yamamoto

  4. Department of Biology, Faculty of Science, Hokkaido University, Kita-ku, Sapporo 060, Japan, , , , , , JP

    N. Morita

Authors
  1. H. Okuyama
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  2. Daisuke Enari
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  3. Akira Shibahara
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  4. Kohei Yamamoto
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  5. N. Morita
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Received: 29 December 1995 / Accepted: 10 April 1996

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Okuyama, H., Enari, D., Shibahara, A. et al. Identification of activities that catalyze the cis-trans isomerization of the double bond of a mono-unsaturated fatty acid in Pseudomonas sp. strain E-3. Arch Microbiol 165, 415–417 (1996). https://doi.org/10.1007/s002030050346

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  • DOI: https://doi.org/10.1007/s002030050346

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