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Molecular characterization of the Sinorhizobium meliloti nlpD gene

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Abstract.

The Sinorhizobium meliloti nlpD gene consists of 1,539 nucleotides and codes for 512 amino acids. Expression of the nlpD gene as a histidine-tagged protein in Escherichia coli resulted in the production of a 57-kDa protein. The deduced polypeptide sequence of NlpD contains one unusual hexamer repeat (KVQRGQ), one tetramer (TVTV) and two direct and inverted trimer repeats (KAA, AAK). The N-terminal amino acid residues displayed similarity with signal peptides of secreted bacterial lipoproteins. Mutations of the S. meliloti nlpD gene caused decreased survival of cells in the stationary phase.

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  1. Institut für Mikrobiologie und Genetik der Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany, , , , ,

    Wolfgang R. Streit, Kai Hofmann & Wolfgang Liebl

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  1. Wolfgang R. Streit
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  2. Kai Hofmann
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  3. Wolfgang Liebl
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Streit, W., Hofmann, K. & Liebl, W. Molecular characterization of the Sinorhizobium meliloti nlpD gene. Arch Microbiol 174, 292–295 (2000). https://doi.org/10.1007/s002030000205

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  • DOI: https://doi.org/10.1007/s002030000205

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