Skip to main content
Log in

Sclerotia degradation by Trichoderma-mycoparasitic; an effective and sustainable trend in the drop lettuce disease control caused by Sclerotinia sclerotiorum

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Controlling the hazard of sclerotia produced by the Sclerotinia sclerotiorum is very complex, and it is urgent to adopt an effective method that is harmonious environmentally to control the disease. Among the six isolates isolated from the rhizosphere of lettuce, the isolate HZA84 demonstrated a high activity in its antagonism towards Sclerotinia sclerotiorum in vitro, and produces siderophore. By amplification of internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF1-α), and RNA polymerase II subunit (RPB2) genes, the isolate HZA84 was identified as Trichoderma asperellum, which was confirmed by analysis of phylogenetic tree. The Scanning electron microscope monitoring detected that the isolate HZA84 spread over the sclerotial surface, thus, damaging, decomposing, and distorting the globular cells of the outer cortex of the sclerotia. The Real-time polymerase chain reaction (RT-qPCR) analysis disclosed the overexpression of two genes (chit33 and chit37) encoding the endochitinase in addition to one gene (prb1) encoding the proteinase during 4 and 8 days of the parasitism behavior of isolate HZA84 on the sclerotia surface. These enzymes aligned together in the sclerotia destruction by hyperparasitism. On the other hand, the pots trial revealed that spraying of isolate HZA84 reduced the drop disease symptoms of lettuce. The disease severity was decreased by 19.33 and the biocontrol efficiency was increased by 80.67% within the fourth week of inoculation. These findings magnify the unique role of Trichoderma in disrupting the development of plant diseases in sustainable ways.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

References

Download references

Acknowledgements

The authors extend their appreciation to the Researchers Supporting Project (RSP2024R120), King Saud University, Riyadh, Saudi Arabia.

Funding

This work was funded by the Researchers Supporting Project (RSP2024R120), King Saud University, Riyadh, Saudi Arabia.

Author information

Authors and Affiliations

Authors

Contributions

All authors contributed to the study conception and design. Conceptualization and methodology were painted by [Ali Athafah Tomah] and [Solabomi Olaitan Ogunyemi]. Data was curated by [Ali Athafah Tomah], [Solabomi Olaitan Ogunyemi], and [Mohamed Mohany]. The investigation was by [Arif Ali Khattak] and [Mohammed Hamdan Aldarraji]. The first draft of the manuscript was written by [Mohammed Hamdan Aldarraji], [Ali Abbas Hashim Al-Maidi], and [Arif Ali Khattak]. Writing-review, and editing were by [Ali Athafah Tomah], [Solabomi Olaitan Ogunyemi], [Mohamed Mohany], and [Salim S. Al-Rejaie]. Resources were collected by [Mohamed Mohany], and [Salim S. Al-Rejaie]. Original drafting and final was written by [Mohammed Hamdan Aldarraji], [Ali Abbas Hashim Al-Maidi], [Mohamed Mohany], and [Salim S. Al-Rejaie]. All authors have read and agreed to the submitted version of the manuscript.

Corresponding authors

Correspondence to Ali Athafah Tomah or Solabomi Olaitan Ogunyemi.

Ethics declarations

Conflict of interest

The authors declare that they have no relevant financial or non-financial interest to disclose.

Consent to participate

All authors have approved the article, including authorship and order of authorship.

Additional information

Communicated by Nischitha R.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tomah, A.A., Khattak, A.A., Aldarraji, M.H. et al. Sclerotia degradation by Trichoderma-mycoparasitic; an effective and sustainable trend in the drop lettuce disease control caused by Sclerotinia sclerotiorum. Arch Microbiol 206, 286 (2024). https://doi.org/10.1007/s00203-024-04014-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00203-024-04014-3

Keywords

Navigation