Abstract
Bacteriocins are ribosomally synthesized bacterial peptides endowed with antibacterial, antiprotozoal, anticancer and antiviral activities. In the present study, we evaluated the antiviral activities of two bacteriocins, enterocin DD14 (EntDD14) and lacticaseicin 30, against herpes simplex virus type 1 (HSV-1), human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero, Huh7 and Vero E6 cells, respectively. In addition, the interactions of these bacteriocins with the envelope glycoprotein D of HSV-1 and the receptor binding domains of HCoV-229E and SARS-CoV-2 have been computationally evaluated using protein-protein docking and molecular dynamics simulations. HSV-1 replication in Vero cells was inhibited by EntDD14 and, to a lesser extent, by lacticaseicin 30 added to cells after virus inoculation. EntDD14 and lacticaseicin 30 had no apparent antiviral activity against HCoV-229E; however, EntDD14 was able to inhibit SARS-CoV-2 in Vero E6 cells. Further studies are needed to elucidate the antiviral mechanism of these bacteriocins.
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Data availability
The data presented in this study are available from the corresponding author on reasonable request.
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Acknowledgements
The numerical calculations reported in this paper were partially performed at TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources).
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The authors (DD, RB, DL, DH) would like to thank la region des Hauts de France for supporting this project through BACOVID project awarded to DD. RT received a fellowship from the same local authority. IE and SD acknowledge Istanbul Kalkinma Ajansi for their support with the Project Number TR10/21/YEP/0133.
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The conceptualization, supervision, project administration, data curation, formal analysis and validation of the study were performed by DD, DH, RB and DL. The investigation and methodology were performed by RT, FS, MPN, IE and SD. Funding acquisition was performed by DD, RB and DH. Manuscript review and editing were performed by all authors. All authors read and approved the final manuscript.
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Communicated by Ran Wang.
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Teiar, R., Sane, F., Erol, I. et al. Enterocin DD14 can inhibit the infection of eukaryotic cells with enveloped viruses. Arch Microbiol 206, 269 (2024). https://doi.org/10.1007/s00203-024-04002-7
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DOI: https://doi.org/10.1007/s00203-024-04002-7