Abstract
This study compared the EXS 2600 system with the MALDI Biotyper for identifying microorganisms in dairy samples. Of the 196 bacterial isolates from milk, whey, buttermilk, cream, and dairy wastewater, the species and genus consistent identification between two systems showed 74% and 99%, respectively. However, the level of species identification rate exhibited a difference, which was higher in Zybio than in Bruker—76.0% and 66.8%, respectively. Notably, the EXS 2600 system performed better with certain yeast species and H. alvei, while the Biotyper excelled with Pseudomonas bacteria. Unique microbial compositions were found in 85% of dairy samples, with whey and buttermilk having the highest diversity. This research highlights the EXS 2600's potential as a reliable dairy microbial identification tool and underscores the need for a more diverse and comprehensive spectral database, despite the database's focus on clinical applications (as announced).
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Data availability statement
All data generated or analyzed in this study are included in this published article (and its Supplementary Information Files).
Abbreviations
- 16S rDNA:
-
16S ribosomal deoxyribonucleic acid
- MALDI–TOF MS:
-
Matrix-assisted laser desorption/ionization–time of flight mass spectrometry
- α-CHCA:
-
α-Cyano-4-hydroxycinnamic acid
- kDa:
-
Kilodalton
- HPLC:
-
High-performance liquid chromatography
- UV:
-
Ultraviolet
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Acknowledgements
M.Z. and P.P. are members of Toruń Center of Excellence “Towards Personalized Medicine” operating under Excellence Initiative-Research University.
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This research was funded by The National Centre for Research and Development grant TANGO-V-A/0014/2021-00.
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Conceptualization, G.C-R., P.P.; data curation, G.C-R., M.R., A.B.F.; formal analysis, M.Z.; funding acquisition, P.P.; investigation, G.C-R., M.R., A.B.F.; methodology, G.C-R., A.B.F., M.R., and M.Z.; project administration, P.P.; resources, P.P.; supervision, P.P.; validation, G.C-R., A.B.F.; writing—original draft preparation, G.C-R.; A.B.F, M.Z., writing—review and editing, A.B.F., M.Z.; project administration, P.P. All authors have read and agreed to the published version of the manuscript.
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Communicated by Yusuf Akhter.
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Czeszewska-Rosiak, G., Złoch, M., Radosińska, M. et al. The usefulness of the MALDI–TOF MS technique in the determination of dairy samples’ microbial composition: comparison of the new EXS 2600 system with MALDI Biotyper platform. Arch Microbiol 206, 172 (2024). https://doi.org/10.1007/s00203-024-03885-w
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DOI: https://doi.org/10.1007/s00203-024-03885-w