Abstract
A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115T, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115T was MK-7. The major cellular fatty acids were anteiso-C15:0 (44.8%), anteiso-C17:0 (20.5%), and iso-C15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l-Lys-Gly-d-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009T (96.5%) and Nesterenkonia lutea YIM 70081T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115T (= MCCC 1K06658T = KCTC 49495T).
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This was was funded by Mingguo Jiang (AA18242026, AD18281066).
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M , conceptualisation and project administration. Contributed to the conception of the study, formulation or evolution of overarching research goals and aims. Y J, resources. Provision of some study materials. A Z, investigation and writing – original draft preparation, performing all of the experiments. H C validation and writing – review and editing, performing most of the experiments. Y H, performed research; Q Y, performed research; Z Z, analyzed data. Y Z, resource.
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Zhao, A., Cai, H., Huang, Y. et al. Nesterenkonia marinintestina sp. nov., isolated from the fish intestine. Arch Microbiol 206, 110 (2024). https://doi.org/10.1007/s00203-023-03825-0
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DOI: https://doi.org/10.1007/s00203-023-03825-0