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Biofilm formation and EPS production enhances the bioremediation potential of Pseudomonas species:  a novel study from eutrophic waters of Dal lake, Kashmir, India

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Abstract

The present study was conducted with the aim of isolation and identification of the biofilm-forming denitrifying Pseudomonas bacterial strains from eutrophic waters of Dal lake, India, followed by the study of inter-relation of biofilm formation and denitrification potential of Pseudomonas strains. The bacterial strains were characterized by morphological observations and identified using 16S rDNA sequencing followed by the quantification of biofilm formation of these st by crystal violet (CV) assay using 96-well microtiter plate and extracellular polymeric substance (EPS) extraction. Lastly, the nitrate-reducing potential of all Pseudomonas species was studied. Our evaluation revealed that four different Pseudomonas species were observed to have the biofilm-forming potential and nitrate-reducing properties and the species which showed maximum biofilm-forming potential and maximum EPS production exhibited higher nitrate-removing capacity. Moreover, P. otitis was observed to have the highest denitrification capacity (89%) > P. cedrina (83%) > P. azotoform (79%) and the lowest for P. peli (70%). These results clearly signify a positive correlation of biofilm-forming capacity and nitrate-removing ability of Pseudomonas species. This study has for the first time successfully revealed the bioremediation potential of P. otitis, P. cedrina, P. azotoform, and P. peli species, thus contributing to the growing list of known nitrate-reducing Pseudomonas species. Based upon the results, these strains can be extrapolated to nitrate-polluted water systems for combating water pollution.

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Data availability

Data about the 16S rRNA gene sequences have been deposited at NCBI GenBank under the accession numbers MW979592, MW979593, MW774332, and MW979605. https://www.ncbi.nlm.nih.gov/nuccore/MW979592,MW979593,MW774332,MW979605.

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Acknowledgements

The authors are highly thankful to Director CORD, previous directors, Department of Environmental Science/Centre of Research for Development (CORD), University of Kashmir, Department of Chemical Engineering, NIT, Srinagar for providing laboratory facilities to carry out the research work and special thanks to Department of Science and Technology (DST), Govt. of India for funding the research work

Funding

The financial support of the research work was provided by the Department of Science and Technology (DST), Govt. of India, under Women Scientist’s scheme (WOS-A) vide reference number SR/WOS-A/LS-232/2018.

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  1. Microbiology Research Laboratory, Centre of Research for Development (CORD)/Department of Environmental Science, University of Kashmir, Srinagar, J&K, India

    Riasa Zaffar, Ruqeya Nazir & Rubiya Dar

  2. Energy Engineering Lab, Department of Chemical Engineering, National Institute of Technology (NIT), Srinagar, J&K, India

    Mushtaq Ahmad Rather

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RZ: Concept, design, collection, conducting experiments, compilation, interpretation of data, writing—original draft, editing, data curation, and formal analysis. RN: Concept, design, interpretation of data, data curation, formal analysis, review and editing. MAR: Concept design, interpretation of data, data curation, formal analysis, review, and editing. RD: Interpretation of data, review and editing. All authors contributed to the article and approved the submitted version.

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Zaffar, R., Nazir, R., Rather, M.A. et al. Biofilm formation and EPS production enhances the bioremediation potential of Pseudomonas species:  a novel study from eutrophic waters of Dal lake, Kashmir, India. Arch Microbiol 206, 89 (2024). https://doi.org/10.1007/s00203-023-03817-0

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