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Anti-microbial efficacy of a scientifically developed and standardized herbal-alcohol sanitizer

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Abstract

Hands are the primary mode of transmission of microbe-based infections, as they harbor normal microbiota and pathogenic microbes. SARS-CoV-2 has endangered lives worldwide, and WHO has recommended good hygiene practices, especially hand hygiene. In addition, other infectious diseases like diphtheria, measles, tuberculosis, HIV, malaria, etc. are spreading in the shadow of the COVID-19 pandemic. The anti-microbial efficiency of two in-house developed herbal-alcohol based hand sanitizers containing Azadirachta indica, Citrus limon, Zingiber officinale, and Aloe vera (HS1) and Zingiber officinale replaced with Ocimum sanctum (HS2) was evaluated. HS1, with Zingiber officinale, and HS2, with Ocimum sanctum, herbal sanitizers showcased in-vitro anti-viral activity on MDCK cells using the reference strain of influenza A virus, A/PR/8/34 (H1N1), and reduced 99.99% of microbial load within 30 s of contact time, estimated by the Antimicrobial Susceptibility Testing Method. On volunteers, HS1 and HS2 were more effective than alcohol-based WHO sanitizers. Moreover, HS2 sanitizer is more effective against viruses and has better efficiency and hedonic qualities in volunteers than HS1. These sanitizers don’t irritate or dry up the skin and have a longer shelf life. Overall, findings reveal that herbal-alcohol-based sanitizers are promising hand hygiene products with the capability of reducing microbial load.

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Data cannot be shared publicly because of Defence confidential data restriction policy. The reasonable data request can be made at: rktulsawani@yahoo.com (Dr. Rajkumar Tulsawani, corresponding author).

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Acknowledgements

The authors are sincerely thankful to the Defence Research and Development Organisation (DRDO), Ministry of Defence, Delhi, India for funding reported studies and the Director, DIPAS for supporting this research work.

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This work was supported by Defence Research and Development Organisation (COVID-19 Emergency Fund).

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Authors and Affiliations

  1. Defence Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, New Delhi, India

    Rajkumar Tulsawani, Kalyani Verma, Ekta Kohli, Purva Sharma, Yogesh Singh Meena,  Amitabh & Rekha Maithani

  2. Defence Research and Development Establishment (DRDE), Jhansi Road, Gwalior, India

    Sarkaraisamy Ponmariappan

  3. Amity Institute of Virology and Immunology (AIVI), Amity University, Sector-125, Noida, India

    Prashant Kumar

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Conception and design of the study: RT; Acquisition and analysis of data: RT, KV, EK, SP, PK, PS, YSM, A, SP, PK; Drafting of the manuscript, tables, and figures: RK, KV, EK. All authors reviewed the manuscript.

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Tulsawani, R., Verma, K., Kohli, E. et al. Anti-microbial efficacy of a scientifically developed and standardized herbal-alcohol sanitizer. Arch Microbiol 206, 77 (2024). https://doi.org/10.1007/s00203-023-03805-4

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