Abstract
Pseudomonas aeruginosa is an opportunistic bacterium that can form a biofilm with the ability to colonize different surfaces and for increasing resistance to antibiotics. An alternative to solve this problem may be the use of non-glucose/mannose glycosylated proteins from Melipona beecheii honey, which are capable of inhibiting the growth of this pathogen. In this work, the antibiofilm activity of the conA-unbound protein fraction (F1) from M. beecheii was evaluated. The crude protein extract (CPE) and the F1 fraction inhibited the P. aeruginosa biofilm growth above 80% at 4 and 1.3 µg/mL, respectively. These proteins affected the structure of the biofilm, as well as fleQ and fleR gene expressions involved in the formation and regulation of the P. aeruginosa biofilm. The results demonstrated that the F1 fraction proteins of M. beecheii honey inhibit and affect the formation of the P. aeruginosa biofilm.
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Acknowledgements
The authors wish to thank Alvaro Morales for technical assistance; Paola Campa for manuscript revision and Dr. Victor Rejon for his technical help in the use of the Scanning Electron Microscope.
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This study was supported by Tecnológico Nacional de México (TecNM) throught the Grant 17678. The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.
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LPY contributed to conceptualization, design of methodology and formal analysis of results. JRS contributed on formal analysis, writing and review the paper. AIO and RZB contributed to the statistical analysis of the results. EOV contributed to conceptualization, designed the study, supervised the laboratory, writing and review the paper. All the authors have read the final manuscript and approved the submission.
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Pool-Yam, L., Ramón-Sierra, J., Oliva, A.I. et al. Effect of conA-unbound proteins from Melipona beecheii honey on the formation of Pseudomonas aeruginosa ATCC 27853 biofilm. Arch Microbiol 206, 54 (2024). https://doi.org/10.1007/s00203-023-03783-7
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DOI: https://doi.org/10.1007/s00203-023-03783-7