Abstract
Duckweed has been highlighted as an invaluable resource because of its abilities to remove nitrogen and phosphorus from wastewater coupling with the production of high starch/protein-containing plant biomass. Duckweed recruits microbes and particularly forms a stable “core” bacterial microbiota, which greatly reduces the colonization efficiency of plant growth-promoting bacteria (PGPB). In this study, natural duckweeds were enriched in a sterilized-partially treated wastewater effluent from a poultry farm. After 24 days of cultivation, the duckweed-associated bacteria (DAB) were isolated and evaluated for their plant growth-promoting (PGP) potentials by co-cultivation with axenic Spirodela polyrhiza. Ten species were found in more than one location and could be considered candidates for the stable “core” DAB. Among them, all isolates of Acinetobacter soli, Acidovorax kalamii, Brevundimonas vesicularis, Pseudomonas toyotomiensis, and Shinella curvata increased duckweed growth in Hoagland medium. The highest PGP ability was observed in Sh. curvata W12-8 (with EPG value of 208.72%), followed by Paracoccus marcusii W7-16 (171.31%), Novosphingobium subterraneum W5-13 (156.96%), and Ac. kalamii W7-18 (156.96%). However, the highest growth promotion in the wastewater was observed when co-cultured with W7-16, which was able to increase biomass dry weight and root length of duckweed by 3.17 and 2.26 folds, respectively.
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The data supporting the findings of this study are available within the article and its supplementary material.
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Funding
This work was funded by the Kasetsart University Research and Development Institute (KURDI) under the project FF(KU)4.64 “Livestock wastewater treatment and biomass production of duckweed using duckweed growth promoting-associated microbes”.
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CB provided conceptualization, funding acquisition, project administration, resources, supervision, data curation, formal analysis, investigation, methodology, writing—original draft, and editing. SK performed the data curation and investigation. BB performed the data curation and investigation. MM provided project administration and resources. KD provided project administration and resources. All authors have read and agreed to the published version of the manuscript.
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Boonmak, C., Kettongruang, S., Buranathong, B. et al. Duckweed-associated bacteria as plant growth-promotor to enhance growth of Spirodela polyrhiza in wastewater effluent from a poultry farm. Arch Microbiol 206, 43 (2024). https://doi.org/10.1007/s00203-023-03778-4
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DOI: https://doi.org/10.1007/s00203-023-03778-4