Abstract
A novel filamentous eel-leptocephalus pathogenic marine bacterium, designated strain EL160426T, was isolated from Japanese eel, Anguilla japonica, leptocephali reared at a laboratory in Mie, Japan. In experimental infection studies on eel larvae, the strain EL160426T caused massive larval mortality and was reisolated from moribund leptocephali. Characteristically, observations of infected larvae found that EL160426T forms columnar colonies on the cranial surface of larvae. The novel isolate exhibited growth at 15–30 °C, pH 7–9, and seawater concentrations of 60–150% (W/V). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EL160426T was most closely related to Aureispira maritima 59SAT with 97.7% sequence similarity. The whole genome sequence analysis of the strain EL160426T showed that the strain maintained a circular chromosome with a size of approximately 7.58 Mbp and the DNA G + C content was 36.2%. The major respiratory quinone was MK-7 and the predominant cellular fatty acids were 16:0, 20:4 w6c (arachidonic acid), 17:0 iso and 16:0 N alcohol. DNA relatedness between the closest phylogenetic neighbor strain EL160426T and A. maritima (JCM23207T) was less than 13%. On the basis of the polyphasic taxonomic data, the strain represents a novel species of the genus Aureispira, for which the name Aureispira anguillae sp. nov. is proposed. The type strain is EL160426T (= JCM 35024 T = TSD-286 T).
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Data availability
The 16S rRNA gene and complete genome sequences have been deposited in GenBank under the accession numbers LC645360 and AP026867 to AP026869 (AP026867 for chromosome, AP026868 for plasmid pAUEa and AP026869 for plasmid pAUEb), respectively.
Abbreviations
- MK:
-
Menaquinone
- JCM:
-
Japan Collection of Microorganisms
- TSD:
-
Type Strain Deposit number in the American Type Culture Collection (ATCC)
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This work was supported by Ministry of Agriculture, Forestry and Fisheries of Japan.
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This work received a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan.
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Yuasa performed the isolation, deposition and polyphasic taxonomy. Mekata performed genome analysis. Kiryu performed histological observations and revised the manuscript. Nomura and Sudo collected infected fish from a rearing tank and performed an experimental infection test. Satomi revised the manuscript. All authors read and approved the final manuscript.
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The care and use of experimental animals was conducted in accordance with the guidelines of the Japan Fisheries Research and Education Agency and the guidelines set by the Japanese Ministry of Environment regarding standards for the care and use of laboratory animals, including the minimization of pain.
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Communicated by Yusuf Akhter.
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Yuasa, K., Mekata, T., Kiryu, I. et al. Aureispira anguillae sp. nov., isolated from Japanese eel Anguilla japonica leptocephali. Arch Microbiol 206, 47 (2024). https://doi.org/10.1007/s00203-023-03771-x
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DOI: https://doi.org/10.1007/s00203-023-03771-x